Study On Corrlation Between Visfatin And Its Expression And Gestational Diabetes Mellitus

Objectives: To observe the changes in serum levels of visfatin and expression of gastrocolic omentum VFmRNA, and to assess their relationship with insulin resistance, metabolic parameters, newborn birth weight in late trimester pregnant women with varying degrees of glucose intolerance.Methods: one hundred and twenty cases of pregnancy women in late trimester were divided to three groups according to their reacts of a 50-g glucose challenge test(GCT) and 3h-75g OGTT: gestational diabetes mellitus group (GDM, n=45),50g glucose challenge test(+) group(GCT+, n=20), and normal glucose tolerance group(NGT, n=55). Fast-blood samples were collected on the day of elective caesarean section, serum visfatin in blood was determined by ELLSA, insulin was measured by chemiluminescent immunoassay(CLIA), glucose determination was carried out by glucose oxidase method, TC,TG were measured by enzymic method , HOMA-IR was used in insulin stable model calculation. Gastrocolic omentum samples were collected from women during caesarean section, the expression levels of VFmRNA were determined by Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR);Newborn birth weight were recorded. Statistical analysis was made by SPSS12.0Results: 1. Higher concentrations of plasma FPG, TG, TC and HOMA-IR were found in GDM group than GCT(+) group and NGT group. Levels of serum visfatin were elevated, accompanied by the degrees of abnormal glycometabolism, but the difference was not statistically significant among those three groups. Visfatin levels were significantly higher in GDM group than NGT group. (P<0.05).2.No significant differences were noted in GCT(+) group with GDM and NGT group in serum visfatin levels.3.Levels of serum visfatin showed no association with expression levels of VFmRNA,metabolic parameters, IR and newborn birth weight.4.Significant differences were found in the expression levels of gastrocolic omentum VFmRNA in those three groups(P<0.05), higher expression levels were detected in GDM group comparing with GCT(+) and NGT group. Furthermore, positive correlation was found in omentum VFmRNA and BMI (r=0.32,P<0.01), but there was no correlation between levels of serum visfatin with insulin resistance, metabolic parameters and newborn birth weight.Conclusions: During the 3rd trimester of pregnancy, serum VF levels and expression of gastrocolic omentum VFmRNA in GDM women had significant difference with GCT(+) and NGT pregnancy women. Serum visfatin levels and its expression increased, following the deterioration of glycometabolism. There was no association between the expression of VFmRNA and IR, metabolic parameters and newborn birth weight besides with BMI. In conclusion, VF was closely correlated with glycometabolism in pregnancy. It is one kind of insulin-like but its action is independent from that of insulin. further study is needed to clarify its mechanism.