Study On Extraction And Immunocompetence Of Averrhoa Caramhola L. Polysaccharides

Plant polysaccharides composed of single sugar orβ-glycosidic bouds, and existed widely in plant kingdom including of amylum, cellulose, polysaccharide and pectin. Many study showed that polysaccharides had pharmacologic action of immunoregulatory, anti-tumour, anti-oxygenation, anti-decrepitude, and so on. Averrhoa caramhola L. had abundant resource and effect of detoxification and colling blood. The study report about extraction of polysaccharides from A verrhoa caramhola L and its applications of immune function were not found at present.Crude Averrhoa caramhola L. polysaccharides was isolated from extracting with hot water and precipitating with ethyl alcohol. Free proteins were removed by multiple enzyme. Averrhoa caramhola L. polysaccharides was obtained from gel filtration chromatography.The primary structure and some properties of the polysaccharide were separately identified by ultraviolet spectrum scanning, KBr infrared spectroscope. The results showed that the extract rate of crude Averrhoa caramhola L. polysaccharides was 3.42%, and total gluose content was 70.5%. Averrhoa caramhola L. polysaccharides was uniform and high purify, it was probably composed of three kinds of sugars.For observing the effect of immunity function of mouse by Averrhoa caramhola L. polysaccharides, foot pad increasing and engulfing carbon granula test were carried, and ratio of organs to weight, thymic factors, and contents of IL-2 and IL-6 were determine. The results showed that compared with the control group, the mid and high dose of Averrhoa caramhola L. polysaccharides could strengthen on mouse weight and ratio of organs to weight of mouse (P<0.05). The mid and high dose of Averrhoa caramhola L. polysaccharides could strengthen on the thymic factors and the immunity function of mouse (P<0.05). The experiment of Effect of Averrhoa caramhola L. polysaccharides tincture on carbon granulation sweeping away of mouse showed that the mid and high dose of Averrhoa caramhola L. polysaccharides could strengthen on tincture on carbon granulation sweeping away of mouse. The mid and high dose of Averrhoa caramhola L polysaccharides could strengthen on on the content of IL-2 and IL-6 of mouse. So the result showed that Averrhoa caramhola L. polysaccharides could strengthen the effect of immunity function to T cells and B cells.