| Both acute myocardial infarction (AMI) and AMI associated congestive heart failure are the main cardiovascular diseases which increase the incidence and morbidity in the modern society. Recently, researchers are trying to transplant stem cells to repair the necrotic myocardial tissue. The curative effect is exciting in some animal and a few clinical experiments. As is confirmed, much cytokine (CK) is secreted when tissue is injury, such as SDF-1, which will mobilize stem cells homing from bone marrow to the ischemic tissue to help repair the injury tissue. It seems exogenous CK administrated in suitable phase can increase the efficiency of stem cells homing and promote the recovery of ischemic tissue.It is focused on that SDF-1 plays a very important role in stem cells mobilizing, homing, adhering, surviving and generating. VEGF is able to promote eothelial cells (EC) to generate as an efficient angiogenesis factor. There are some reports confirming SDF-1 and VEGF expression is increased in plasma and infarct myocardial tissue after MI. Regretfully, no report mentioned the examination in both plasma and infarct myocardia.In our study, AMI model is established in Wistar rat. By the method of immunohistochemistry, SDF-1 and VEGF expression is analyzed qualitatively in protein level on the first day, third day, seventh day, forth day after AMI respectively. Meanwhile, by the method of RT-PCR, they are analyzed qualitatively and semiquantitatively in mRNA level. Dynamic change of SDF-1 and VEGF after AMI is observed to illuminate the CKs'role in the course of infarct myocardia repairing. As basic experiments before clinical application, When to apply SDF-1 and VEGF to clinical is implicated according to our research.Method:AMI model is established in Wistar rat, who were killed in first, third, seventh and fourteenth day randomly. SDF-1 and VEGF were stained by HE and SP. By the method of RT-PCR, both of them were analyzed semiquantatively.Results:Observation of SDF-1 and VEGF expression by the method of immunohistochemistry:1 SDF-1 protein expression:SDF-1 protein expression was observed in the myocardia of infarct zone and border zone on the 1st day and 3rd day in AMI group. While, on the 7th and 14th day, no expression was observed in infarct center but in border zone. SDF-1 protein seemed absent in normal zone of AMI group and shame operation group.2 VEGF protein expressionVEGF protein expression was observed in the myocardial cells of border zone but not in infarct center zone on 1th day and 3th day in AMI group. While, on the 7th and 14th day, no expression was observed in myocardial cells of both infarct center zone and border zone. On the 3th day, weak expression was observed in vessels of suturing zone in shame operation group, but not on the 7th and 14th day.Observation of SDF-1 and VEGF expression by the method of RT-PCR:1 SDF-1 mRNA expression:SDF-1 mRNA expression increased to peak on the first day after AMI and decreased in the other time points sharply.2 VEGF mRNA expression:VEGF mRNA started to express on the 3rd, 7th and 14th after AMI and peeked on the 7th day. The peek lasted to the 14th day after AMI.Conclution:1 SDF-1 wasn't expressed in normal myocardia. SDF-1 expression in infarct center and border zone increased to leak on the 1st after AMI, which decreased sharply later. Till 7th day, the expression was absent in infarct center zone but lasted 14 days in border zone at least.2 VEGF wasn't expressed in normal myocardia. VEGF expression started to incease in the border zone on the 3rd day and peaked on the 7th day after AMI, which lasted much longer. 3 SDF-1 and VEGF were expressed in different time point.
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