The Expression Of Skp2 And P27^kip1 In Colon Carcinoma

Objective: To investigate the expression and correlation of Skp2 and P27^kip1 in colon carcinoma and detect the relationship with the development of the diseases.Method: To detect the protein expression of Skp2 and P27^kip1 by imrnunohistochemistry staining (SABC method) in 50 cases of colon cancer and 20 normal colonic mucosas. The result, which estimate according to the intension of stain, Was classified into 0-3 scores. 0 (no colored), 1 score (slight), 2 scores (moderate), 3 scores (strength). The stain extent, which according to the percent of positive cells, was divided into 0-3 scores. 0 (0%), 1 (<25%), 2(25-50%), 3 (>50%) . The final scores were obtained by adding extent of stain score and intension of stain score together. The final score which was less than 2 was negative cases, the positive cases was more the 2. statistical analysis made by spss11.0 software according to clinicopathological data of colon cancer.Result: the positive expression of Skp2 and P27^kip1 mainly located within the cellular nucleus. The protein expression of skp2 was 64.0%, significantly higher than normal colonic mucosa. 15.0% (P<0.05). The protein expression of P27^kip1 was 22.0% in the colonic cancer, significantly lower than normal colonic mucosa. 90.0% (P<0.05). Both expression of Skp2 and P27^kip1 have negative relationship with age and sex. But they have the positive relationship with the differentiation, Dukes staging and lymph node metastases (P<0.05). The negative correlation was present between the Skp2 and P27^kip1( r=-0.282, P<0.05).The positive expression of p53 mainly located within the cellular nucleus and was brown. There is no expression in the normal colon tissue (0.0%, 0/20) but there was significant increased expression in the colon cancer (80.0%, 40/50, P<0.01) . The positive express of the bcl-2 gene , which located within cytoplasm, can be detected both in the normal colon and colon cancer. There was a significant increase of positive expression of bcl-2 in the colon cancer 74.0% (37/50) compared with the normal tissue 10.0% (2/20) (P<0.05). The expressions of both p53 and bcl-2 have the significant relationship with the pathologic differentiation of colon cancer, but have no relationship with age, sex, stage and lymph metastasis. Skp2 has positive correlation with p53, bcl-2 respectively(r =0.345, r=0.231,P<0.01). Conclusion: The abnormal expression of both Skp2 and P27^kip1 is one of the reasons that cause the development of colon cancer. It may be realized through the up-regulate the expression of p53 and bcl-2. The abnormal expression of Skp2 and P27^kip1 has special relationship with the differentiation, Dukes staging and lymph node metastases of colon cancer respectively.