The Inhibition Effects Of Okinawa Habu Apoxin Protein-1 On Rat Subcutaneous Transplanted Glioma

Glioma is a type of primary central nervous system (CNS) tumor that arises from glial cells. The most common site of involvement of gliomas is the brain, but they can also affect the spinal cord or any other part of the CNS, such as the optic nerves.The morbidity of glioma is the highest in primary brain tumor, and cannot cure it compltely. Surgery is the best way to cure it, but glioma are biologically aggressive, and always invade adjacent tissues, so it is difficult to remove it completely. The glioma is easily recrudescence. Now the gene therapy and inducing apoptosis drugs maybe the good way to cure it. Among these therapies, the snake venom preparation was proved that it has antitumor effect by vivo and vitro experiment. The snake venom preparation is becoming the popular research in antitumor.Objective:To explore the effect of Okinawa Habu Apoxin Protein1 (OHAP-1) from Okinawa Habu snake venom on apoptosis in rat subcutaneous transplanted glioma and its mechanism. It will provide scientific reference for development of antituor drugs.Methods:The glioma model was made by injection of C6 glioma into subcutaneous tissue. There are two groups in this study, including control group and OHAP-1 treatment group. The control group inject PBS into the site of glioma, and the OHAP-1 treatment group inject OHAP-1 into the site of glioma. The size and weight of glioma is mesured.TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labeling (TUNEL) methods were used to investigate whether OHAP-1 induces the apoptosis or not. The TUNEL positive cells were measured and photographed using a confocal laser scanning microscope. The HE stain was used to test the change of tumor. The immunohistochemistry was used to detect the proliferation of the tumor.Results:Tumor growth inhibited after 5 days treatment of OHAP-1. In addition, the apoptic index of tumor tissues assessed by the TUNEL method was significantly increased. The HE stain showed that tumor angiogenesis decreased in rat after 7 days treatment with OHAP-1.The immunohistochemistry ofα-SMA showed that the number of smooth muscle in tumor also decreased in rat after 7 days treatment with OHAP-1.Conclusions:1.Okinawa Habu Apoxin Protein1 (OHAP-1) from Okinawa Habu snake venom can inhibit tumor growth in rat subcutaneous transplanted glioma.2.The mechanism may involved in that OHAP-1 can induce apoptosis of tumor and inhibit angiogenesis of tumor.3.OHAP-1 is a potential antitumor drug.