Empirical Study Of Immunoloregulation By Estrogen After Hepatic Impact Injury

The pyemia and multiple organ dysfunction caused by immune suppression after severity trauma are the nodus of clinic remedy and the main death causing, precaution and nonage management are the key point of clinic remedy.There are difference ambisexuality in immune response and susceptivity to susceptivity, hint that estrogen have something to do with the maintainance and accommodation of immune system.Recent research discover there is also sex differences in trauma。In this study, after estrogen-inject and the models of LII(Liver Impact Injury) in animals were established, we investigate immunoloregulation of estrogen after trauma and the possible mechanism,to provide experimental evidence for clinic remedy of trauma.Methods:Eighteen rabbit were divided randomly into 3 groups, Group A: simple impact group; Group B: normal dosage estrogen- impact group (benzestrofol 1mg /Kg); Group C: large dosage estrogen- impact group (benzestrofol 5mg /Kg). Hepatic impact injury was established with BIM-Ⅳbio-impactor at appendix ensiformis. Detected TNF-a, IL-6, IL-8, Neutrophils infiltration(using myleoperoxidase activity ,MPO),and determine liver tissues estrogen receptors express by immunohistochemistry.The main outcomes of this research are:1. The model of LII in animals has the advantages of simple manipulation of impacting equipment, controllable physical parameters and accuracy and repeatability in impacting sites. The pathology, type and wound state of the LII in the animals are similar to those seen in clinical practice. They are in the range of gradeⅢto grade V according to AAST grading standards and 3~5 points in accordance with the AIS scoring. And LII is complicated with costal fracture and injury of the heart and lungs,conform to the stander of multiple injury. The model can be used for analysis of dynamic factors, detection of many indices. The gross anatomy, death rate and death rate change had no significant variability in three group. 2. In the animals complicated with pulmonary injury, the hepatocytic emboli were occasionally seen in small arteries in the lungs. This finding suggests that hepatic cells exfoliated and reached the pulmonary blood vessels with the blood circulation to form emboli when the liver is impacted to rupture. It is of important value for clinical practice for it is helpful for the further understanding of the histopathological characteristics of LII in complication with pulmonary injury.3.There is no difference of serum TNF-a,IL-6,IL-8,liver tissue MPO in three groups before LII, indicate that pre-injection of estrogen has no affection on basic levels of serum TNF-a,IL-6,IL-8,liver tissue MPO .After LII, serum TNF-a,IL-6,IL-8,liver tissue MPO were lower in group B and group C than in group A(p<0.01), and group C was lower than group B(p<0.05).So,we can conclude that estrogen can dose-dependent attenuated the serum TNF-α,IL-6,IL-8 level,and weaken the aggregation and activation of neutrophi,to relieve immune suppression after trauma. Estrogen can be a novel immunoreguation adjunct for posttraumatic male.4. There is no difference of serum E2 and liver estrogen receptor expression before or after LII. After LII,serum E2 and liver estrogen receptor expression were higher in group B and group C than in group A(p<0.01), and group C was higher than group B(p<0.05). Indicate that trauma itself have no effection serum E2 and liver estrogen receptor expression , estrogen injection before trauma could elevate serum E2, liver estrogen receptor expression also heighten at the same time .We can conclude that the immunoreguation of estrogen mainly mediated by elevated target organ estrogen receptor and immune cells.