Generation Of Progenitor Hair Cells By Differentiation Of Bone Marrow Mesenchymal Stem Cells

objective :To generate of progenitor hair cells by differentiation of mouse bone marrow mesenchymal stem cells.Methods: The bone marrow stromal cells of mice were isolated by anchoring culture, passaged three to five times before being used for experiments.The cells were cultured in DMEM/F12+N2+EGF+ bFGF for 5 days induced differentiation into neural stem cells (Nestin positive,checked by hischemistry) then cultured in DMEM/F12+N2+IGF+EGF+RA for 18 days followed by medium (DMEM/F12 +1%N2) alone for 7 days. RT-PCR were performed to determine the expression of cochlear hair cell markers (myosin VIIa and Math1 )in these differentiated BMSCs.Results : Originally, BMSCs were small, round, or spindle-like in morphology, cultured for 5 days with DMEM/F12+N2+EGF+bFGF,the volume of cells increased and some appeared with prominences. BMSCs became neuron-like cells or epithelial cell-like cells when induced by DMEM/F12+N2+IGF+EGF+RA, BMSCs express Nestin after treated with EGF+bFGF for 5 days. Math1 and myosin VIIa were expressed after futher treating .Conclusions: Bone marrow mesenchymal stem cells can be induced to differentiate into neural stem cells and progenitor hair cells. BMSCs could be the resources of cell implantation for sensory deafness therapeutic use .