| Objective To explore the effect of tendon-bone healing when allogenic tendon graft was wrapped with autologous periostem around the tendon in rabbits.Methods Twenty healthy New Zealand white rabbits were used in the experiment. A one-side posterior limb was selected randomly as a test, and the contralateral limb was served as a control at the same time. The allogenic tendon graft was designed as tendon-bone model in the proximal tibial metaphysis of rabbits. The portion of tendon in the bone tunnel was wrapped with autologous periosteal graft of which the cambium layer was facing toward the bone tunnel on the experimental group. While the portion of tendon in the bone tunnel was not wrapped with autologous periosteal graft on the control group. Histologic examination of the tendon-bone interface and biomechanical test for maximal pullout load were evaluated at 4 and 8 weeks after operation.Results Four weeks after operation, there was significant osteochondral ossification between the tendon graft and the bone tunnel in the experimental group, but little bone appeared in the control group. Another four weeks later, the amount of the newborn bone and the tight interdigitation between the tendon graft and the newly formed bone in the experimental group were superior to the control group. The result of biomechanical testing, according to a paired t test, there was significant difference in statistics between the study groups after 4 and 8 weeks (P＜0.05). According to one-way analysis of variance, there was significant difference in statistics at different time points within each group (P＜0.05).Conclusion The histologic and biomechanical studies in rabbits demonstrated that autologous periosteal graft was wrapped around allogenic tendon graft may shorten the time of osteochondral ossification between the tendon graft and the bone tunnel, improve healing strength and promote the tendon-bone healing in the bone tunnel. |
PDF Full Text Request