| Aims The present study was to examine the effect of Telmisartan to insulin resistance of rats fed high fructose diet and to probe its mechanism.Methods Male SD rats were fed with high fructose diet for 4 weeks to induce IR rats model.All rats were randomly divided into the following groups :C group, TM group,HFD group, HFD+TM group. Then tested the following indicators : (1) Systolic blood pressure(SBP)were measured after fasting overnight, subsequently,blood samples were withdrawn from the end of tails of animals for fasting blood glucose (FBG),fasting serum insulin (FSI)at the 0,2,4,6,8,10,12th weekend and insulin sensitive index (ISI) were calculated. (2) At the 4,12th weekend ,intraperitoneoclysis glucose tolerance test (IPGTT),insulin tolerance test (ITT) were taken.(3) At the 4,12th weekend,fasting blood lipids were measured.RT-PCR detected PPARγ,AT1R,IRS-1,GLUT4 and APN mRNA expression in perirenal fat.(5) At the 4,12th weekend,Immunohistochemical detected AT1R,IRS-1,GLUT4 expression in the lower abdomen fat.Results1.Telmisartan can reduce the level of SBP, FBG ,FSI and improve IR,abnorm lipid metabolism and glucose tolerance test,insulin tolerance test ( P〈0.01 ) .2.Telmisartan can significantly enhance PPARγ, IRS1,GLUT4,APN mRNA expression in perirenal adipose tissue of IR rats(P〈0.01),and reduct AT1R mRNA expression in perirenal adipose tissue of IR rats ( P〈0.01 ) . 3.Telmisartan can significantly enhance IRS1,GLUT4 expression in the lower abdomen fat of IR rats(P〈0.01),and reduct AT1R expression in the lower abdomen fat of IR rats(P〈0.01).Conclusion1.Telmisartan can effectively improve the insulin resistance of the rats fed with high-fructose diet.2. The effects of telmisartan on insulin resistance may be about with it can reduct AT1R expression and increase the expression of PPARγ,IRS1, GLUT4 ,APN in adipose tissue.
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