Relativity Of MTA1 BRMS1 Expressing In Breast Cancer And Relation Of HDAC Activity

Objective:Overpass researching relativity of MTA1,BRMS1 expressing in breast cancer cell and relation of HDAC activity.Try hard to illuminating mechanism of MTA1,BRMS1 operating in breast cancer metastasis.Methods:Surveying gene expression of MTA1,BRMS1 using RT-PCR technique in tree train breast cancer cell individual plant(non-metastasis breast cancer cell plant MCF-7,low metastasis breast cancer cell plant MDA-MB-453,high metastasis breast cancer cell plant MDA-MB-231)Measuring protein . expression of MTA1,BRMS1 with Western blot technique in tree train breast cancer cell individual plant.Contemporary ,surveying HDAC activity byenzyme linked immunosorbent assay in tree train breast cancer cell individual plant.Results⑴Gene expression of MTA1: Gene expressive ratio of MTA1 is 1∶1.9∶3.8 in tree train breast cancer cell stub.The gene expression of MTA1 in the high transfering mammary glands cancer cell stub increased 280% than in those without transfering mammary glands cancer cell stub, Gene expressive quantity of MTA1 increase in evidence following metastasis degree heighten in tree train breast cancer cell stub (P<0.001).⑵Gene expression of BRMS1: Gene expressive ratio of BRMS1 is 3.1∶2∶1 in tree train breast cancer cell stub . The gene expression of BRMS1 in the high transfering mammary glands cancer cell stub decreased 210% than in those without transfering mammary glands cancer cell stub,Gene expressive quantity of BRMS1 reduce in evidence following metastasis degree heighten in tree train breast cancer cell individual plant(P<0.001). ⑶protein expression of MTA1: protein expressive ratio of MTA1 is 1∶2.2∶3.6 in tree train breast cancer cell stub . The protein expression of MTA1 in the high transfering mammary glands cancer cell stub increased 260% than in those without transfering mammary glands cancer cell stub,protein expressive quantity of MTA1 increase in evidence following metastasis degree heighten in tree train breast cancer cell stub (P<0.001).⑷protein expression of BRMS1: protein expressive ratio of BRMS1 is 3.2∶1.9∶1 in tree train breast cancer cell stub . The protein expression of BRMS1 in the high transfering mammary glands cancer cell stub lessened 220% than in those without transfering mammary glands cancer cell stub,protein expressive quantity of BRMS1 reduce in evidence following metastasis degree heighten in tree train breast cancer cell stub (P<0.001).⑸HDAC activity :HDAC activity ratio is 1∶1. 8∶2.6 in tree train breast cancer cell stub . HDAC activity in the high transfering mammary glands cancer cell stub increased 160% than in those without transfering mammary glands cancer cell stub,HDAC activity higher following metastasis degree heighten in tree train breast cancer cell individual plant(P<0.001).Conclusion⑴Gene and protein expressive quantity of MTA1 increase in evidence following metastasis degree heighten in tree train breast cancer cell stub.⑵Gene and protein expressive quantity of BRMS1 reduce in evidence following metastasis degree heighten in tree train breast cancer cell individual plant.⑶expression of MTA1 BRMS1 assume negative relativity in tree train breast cancer cell individual plant,may be a paie positive negative controlling gene.⑷HDAC activity assume increase degree trend following metastasis degree heighten in tree train breast cancer cell individual plan,to cue MTA1 BRMS1 may be regulate breast cancer metastasis via HDAC activity in mechanism of breast metastasis.