| Objective:To compare the incorporation method of 3H-TdR and 125I-UdR on determining the proliferating effect of lymphocyte and lymphoma cell,then evaluate the possibility that 125I-UdR is in place of 3H-TdR as the tracer agent. To analyze the factors which affect the uptake of 125I-UdR and investigate the killing effect of 5-[125I]iodo-2'-deoxyuridine(125I-UdR) in human lymphoma cell line. To evaluate the possibility of treating human lymphoma with 125I-UdR.Method:①125I- UdR was synthesized by nitric acid oxidation method.②The tracer agents of 3H-TdR and 125I-UdR were incorporated into the lymphocytes and lymphoma cells, then the incorporating fraction of the lymphocyte and lymphoma cells were estimated.③The amount of 125I-UdR in the cells and caryons was determined after incubation of different time in RPMI1640 culturing medium containing different concentrations of 125I-UdR. The killing effects of 125I-UdR on Raji and Daudi were estimated through MTT experiment assay. The influence of 125I-UdR on the cell-cycle and cell apoptosis was explored by flow-cytometry(FCM).Results:①The radiochemical purity of 125I-UdR was 99.63%±0.06%, which was still to 98.25%±0.04% after being stored 60 days at 4℃.②The incorporating fraction of 3H-TdR and 125I-UdR into lymphocyte was 20.95%±1.06% and 1.00%±0.04%(±s);The incorporating fraction of 3H-TdR and 125I-UdR into the lymphoma cells was 29.94%±4.10% and 6.02±0.73%. The incorporation fraction of 3H-TdR into lymphocyte and lymphoma cells was much higher than that of 125I-UdR (P<0.01). The incorporating fraction 3H-TdR or 125I-UdR into the lymphoma cells was much higher than that of lymphocyte (P<0.01).③The amount of 125I-UdR in Raji and Daudi cells and caryons was much higher than that of Na125I (P<0.01); The uptake of 125I-UdR in Raji and Daudi cells and caryons increased with the increasing of 125I-UdR concentration and incubated time; The cell surviving fraction of 125I-UdR groups is much lower than that of Na125I groups (P<0.01), and the surviving fraction of 125I-UdR groups reduced with the increasing of 125I-UdR concentration. Conclusion:①The radiochemical purity of 125I-UdR was very high, the stability was fine in vitro, 125I-UdR was suit for being stored at 4℃.②The incorporation fraction of 3H-TdR into lymphocyte and lymphoma cells was much higher than that of 125I-UdR (P<0.01). The incorporating fraction of 3H-TdR or 125I-UdR into the lymphoma cells was much higher than that of lymphocyte (P<0.01).③125I-UdR can be incorporated into DNA of lymphoma cells and kill the cells. The uptake of 125I-UdR is dose dependent and time dependent. 125I-UdR has wildely value and the prospect in clinical application.
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