A Study On The Relationship Between The Expression Level Of WIF-1,Gsk-3β And Nm23-H1 And Prognosis In The Patients With Non-small Cell Lung Cancer

Background and objective Lung cancer is one of the most malignantcancers threatening people's health and life and being the most rapidincreasing both in morbidity and mortality. Tumor metastasis is not only themalignant marker and characteristics of lung cancer, but also the main causeof failure to cure and lose their life of the patients with lung cancer. Atpresent, it has been proved that the abnormality of Wnt signal pathway wasclosely correlated with the oncogensis and development of malignant tumor.WIF-1 and Gsk-3βare the key negative regulators of Wnt signal pathway.WIF-1 (Wnt inhibitory factor-1) can suppress Wnt signal pathway bycompetitive binding of Wnt proteins. Gsk-3β(glycogen synthase kinase-3β)can promoteβ-catenin degradation by phosphorylating it, so the transductionof Wnt signal would be suppressed. Nm23-H1 is an important tumormetastasis suppressor gene, a lot of research results have proven that thereduced expression and deletion of nm23-H1 gene was closely correlated with the oncogenesis and metastasis of many malignant cancers. Theobjective of this study is to investigate the expression and difference ofWIF-1, Gsk-3βand nm23-H1 in non-small cell lung cancer and benignpulmonary lesion, and to study the relationship between their expressions andthe clinical-pathological characteristics, and also to study the relationshipbetween their expressions and the lung cancer patients' prognosis.Methods The expressions of WIF-1, Gsk-3βand nm23-H1 weredetected in 101 non-small cell lung cancer and 30 lung benign lesion tissueby LSAB immunohistochemical stain.Results (1) The positive expression rates of WIF-1, Gsk-3βandnm23-H1 in non-small cell lung cancer were significantly lower than those inpulmonary benign lesion tissues(48.5±24.5% vs. 70.5±25.7%), (68.5±18.8%vs. 88.0±6.1%), (52.0±23.8% vs. 72.0±25.7%), (P＜0.05). (2) Theexpression level of WIF-1, Gsk-3βand nm23-H1 was not related tohistological classification, P-TNM stage and lymph node involvement of thecancer and the age and sex of patients (P＞0.05), but the expression ofnm23-H1 was closely related to the differentiation degree of the tumor. Thelower the differentiation was, the lower the expression of nm23-H1 was(P＜0.05). (3) The WIF-1 expression was highly positive correlated with theGsk-3βexpression in non-small cell lung cancer (r=0.300, P＜0.05), theWIF-1 expression was highly positive correlated with the nm23-H1expression (r=0.612, P＜0.05), the Gsk-3βexpression was also highlypositive correlated with the nm23-H1 expression (r=0.405, P＜0.05). (4)The5-year survival rates of patients with high expression of WIF-1 weresignificantly higher than those of patients with low expression (36.8% vs. 9.1%, P＜0.05), the 5-year survival rates of patients with high expression ofGsk-3βwere significantly higher than those of patients with low expression(29.1% vs. 0, P＜0.05), the 5-year survival rates of patients with highexpression of nm23-H1 were significantly higher than those of patients withlow expression (32.8% vs. 14.0%, P＜0.05).Conclusion (1) The low expression of WIF-1, Gsk-3βand nm23-H1exist in non-small cell lung cancer, and the expression levels of nm23-H1 isclosely related to the differentiation degree of non-small cell lung cancer. (2)A highly positive correlation is existed among WIF-1, Gsk-3βand nm23-H1expression. (3) Detection of WIF-1, Gsk-3βand nm23-H1 expression ishelpful to predict the prognosis of patients with non-small cell lung cancer.