The Expression Of COX-2,VEGF In Huaman Cerebal Saccular Aneurysm And Its Significance

Objective: 1.To observe the tissue pathology and structure change in human cerebal saccular aneurysm. 2.To detect the the expression of C0X-2,VEGF in human cerebal saccular aneurysm and explore the pathogenesis of aneurysm.Methods: Thirty-four surgical specimens of human cerebal aneurysm from 12 males and 22 femals in Huaxi Hospital Sichuan University during 2005.11～2006.10. were choosen. All specimens were got from dissecting after clipping the aneurysm in craniotomy with the guarantee of protecting patient and operation safty. Ten control specimens included 2 cases suferficial artery, cortex artery 6, 1 anterior cerebral artery and 1 middle cerebral artery. These specimens came from hematoma removal and internal decompression. l.To observe the all spicimens with naked eyes and lightscope. 2.To detect the expression of COX-2,VEGF in huaman cerebral saccular aneurysm with sp immunohistochemical method.Results: 1.Naked eyes obervation: Most of the specimens were purple, dark blue or brown, Atherosclerosis plaques could be seen in some of the aneurysmal specimens. There were thrombosis in some cavities of the aneurysms, some were soft, some crisp. Chronic bleeding could also be seen. The wall of the aneurysm varied, the bottom was thicker than the top. Sometimes, only a thin membrane at the top of the aneurysm was left, even ruptured. 2.Lightscope observation: (1)HE staining: All three elements of the aneurysmal wall were damaged totally or partially. The wallof the vessels were abnormal. The thickness of aneurysmal wall was various, the all layers or local region became thin and extended outside, the endothelial cells were bulb degeneration or necrosis. Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae. (2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels. The aneurismal wall were all disordered collagen fibrils. Some collagen fibrils could be found inside the wall. The controlled group indicated low expression or lacked. The difference was statistically significant. The positive rate in internal layer was lower than middle and external layers. No statistical significance. 3.The sp immunhistochemistry staining: The high expressin of COX-2 in human cerebal saccular aneurysmal walls comparing the low expression of in controlled group indicated the statistical significance. COX-2 expressed in internal, middle and external membrane. No statistical significance anong them. The high expressin of VEGF in human cerebal saccular aneurysmal walls comparing the low expression in controlled group indicated the statistical significance. VEGF expressed in internal,middle and external membrane, but there was no statistical significance.Conclusion: 1.The main chang in human cerebal saccular aneurysm lies in the damage or dissappear if the internal elastid menbrane and elastic fibrils, the dissoving of collagen fibrils, the decreasing or dissappeared soomth muscle cells, the inflammatory cells invasion, which leads to the severe damage of matrix layers, decreased the resistant tension. 2.The high expressin of COX-2 in human cerebal saccular aneurysmal walls comparing the low expression of in controlled group indicated the statistical significance. COX-2 expressed in internal, middle and external membrane. No statistical significance anong them. 3.The high expressin of VEGF in human cerebal saccular aneurysmal walls comparing the low expression in controlled group indicated the statistical significance. VEGF expressed in internal, middle and external membrane, but there was no statistical significance.