The Effect On Angiogenesis Of Endometrium After Transcervical Resection Of Polyp

Object: To investigate the effect on angiogenesis of formation of endometrial polyp(EP) and the effect on angiogenesis of endometrium after transcervical resection of polyp. To find the possible mechanism of the molecular pathogenesis of abnormal uterine bleeding and molecular mechanism of transcervical resection of polyp.Methods: 25 patients were chosen whose main symptoms were abnormal bleeding of their uterus, all of their final diagnosises are EP depending on methods of the hysteroscopy and pathology. They all conceived the treatment of transcervical resection of polyp in one day of 5-7 days after the ending of menstruation.The tissue of EP were resected in the operation were collected as the first group. The second group included 20 patients who conceived the treatment of transcervical resection of polyp, in the 1-3 months after the operation ,we collected their endometrium located in the extent of rescection samely in one day of 5-7 days after the ending of their menstruations. 25 endometrium in proliferative stage were collected as the third group. expression of vascular endothelial growth factor (VEGF) and CD34 were detected by immunohistochemical methods. We counted the microvessel density (MVD) depending on microscope, and detected integrated optical density (IOD) of VEGF by using special image analysis software (image pro plus 4.5).Results: (1) IOD of VEGF expression and MVD of the first group were significantly higher than that of the second and the third group(P < 0.05). (2) IOD of VEGF expression and MVD were both same in the second and third group(P>0.05).Conclusion : Significance of the angiogenesis in the formation of EP was obvious. Expression of VEGF and MVD could be degraded in someone's local endometriun after they conceived the treatment of TCRP. According to our research ,we could suppose that these results were one of the molecular mechanisms how TCRP could cure EP and eliminate the patients' symptom of abnormal bleeding of uterus successfully.