Effects Of Cocaine And Amphetamine Regulated Transcript Protein Vaccine On Conditioned Place Preference Induced By Morphine In Mice

Abjective: To study effects of the CART (Cocaine and Amphetamine Regulated Transcript ) protein vaccine on CPP (conditioned place preference) induced by morphine in mice .Method: Expressing plasmid of GST-CART55.102 was constructed . The protein was purified by affinity purification. The effects of the protein vaccine was observed by CPP model .CART antibody was detected by ELISA. Result:Immunization (twice) before conditioned the protein vaccine inhibited acquisition or reacquisition of CPP induced by morphine in mice. Immunization (once) after conditioned the protein vaccine inhibited reacquisition of CPP. Conclusion: GST-CART protein vaccine had the effects of inhibited acquisition or reacquisition of the CPP induced by morphine in mice .The vaccine could cure the drug addiction .