| Disinfectants are usedto kill the pathogenic microorganisms on the media.Skin disinfectants are used tokill pathogenic microorganisms on skin. For skin disinfection agents there are special requirements tostrictly choice. Now we always use chemosterilant which is attenuated can be used on skin. Chemosterilanthas good disinfection,but it can not be used on skin directly and the flavour is very incentive.Investigatorsdeveloped some disinfectants that was composed of traditional Chinese drug or Lactobacillus.No onestudied to combine traditional Chinese drug with Lactobacillus. The effective of bacteriostasis are verywell,there are no abnormal flavour and no incentive, drug resistant strain can not be occurrenced forChinese herbs and Lactobacillus.We try to develop a skin disinfectants which combined traditional Chinesedrug with Lactobacillus fermentation, detect the effective of disinfection and anti-infection.We determined the growth curve and pH curve of Lactobacillus and Bifidobacterium. The growingnumber of Lactobacillus is 4-18h, after 18h entering basic stable, pH down to 3.68. Bifidobacterium of thisexperiment has longer lag, the growing number of post-vaccination is 12h-24h, after 24h pH down to4.51. Using dish drilling method for the determination of Lactobacillus and Bifidobacterium in vitroantibacterial activity. Lactobacillus and Bifidobacterium bacteria without inhibition Zone on its in vitroantibacterial effective. The difference of Lactobacillus (Bifidobacterium) fermentation and fermentationsupernatant liquid in antibacterial effect is not significant. Lactobacillus fermentation supernatant has goodthermal stability. So choice well inhibitory effect and stability lactobacillus supematant as a disinfectantsome probiotic fermentation product representatives.We chose eight Chinese herbs: Flos Caryophylli, Flos Lonicerae, Radix et Rhizomarbei, FructusSchisandrae,Radix Scutellarise,Cortex Phellodendri, Fructus Forsythiae,Fructus Mume. Extraction thseseight Chinese herbs for water or ethanol. Most Chinese herbs extract (extract water and alcohol extract) havestrong inhibition in Staphylococcus aureus than E. coli Inhibition. Water extract of Flos Caryophyliiinhibited E. coli bacteria diameter of 19.5ram. Water extract of Radix et Rhizomarbei inhibitedStaphylococcus aureus bacteria diameter of 20.4mm.Selected more than tow Chinese herbs from Flos Caryophylli. Radix et Rhizomarbei, Radix Scutellarise,Fructus Mum.to extract with water. Selected more than tow Chinese herbs from Flos Caryophylli.Radix etRhizomarbei,Fructus Schisandrae extract with alcohol. With lactic acid fermentation supernatant groupcomposed of six parties. (Flos Caryophylli+,Fructus Schisandrae)water extract+lactobacillus supernatantof E. coli and Staphylococcus aureus inhibitory effect than the other five groups are a good side,the inhibition zone diameter of 22.2mm and23.7mm.The pH of that prescription is 5.41, within normal human skin pH4.0-6.0.Determination of the in vitro antibacterial disinfectant curve. It will kill all E. col In 8h i, and will be ableto kill Staphylococcus aureus 7h. Using orthogonal test to select neutralizer of disinfectant. The bestcombination of 0.5ï¼…lecithin, glycine 1.0ï¼…, 5.0ï¼…Tween 80,0.5ï¼…sodium thiosulfate. Disinfectants on E.coli and Staphylococcus aureus role 2min killing rate could reach 99.99ï¼…. After administration hasrepeatedly skin irritation response was less than the average score of 0.49. Staphylococcus epidermidisinfections establish an animal modell to note disinfectant has good anti-infection. Skin disinfectantdisinfection field test, to note disinfectants meet the requirements.Through those study, Proved that the development of the test is a disinfectant with effective disinfection,irritating small, mild disinfectant. Avoiding the chemical disinfectants of larger irritating smell, not directlyfor skin disinfection and other shortcomings, expanding the using of Lactobacillus. It is effective to useChinese herbs.
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