The Mode And Effects Of Allogeneic Bone Marrow Mononuclear Cell Transplantation In Diabetic NOD Mouse

Objective To find the method and the implantation evidence of bone marrow mononuclear cell(MNC)transplantation in diabetic NOD mice.Method Fifteen NOD mice developing into type 1 diabetes mellitus were divided randomly into three groups:Sublethal dose radiation group(750cGy),lethal dose radiation group(950cGy),and control group.NOD female recipient mice were irradiated with Sublethal dose 750 cGy or 950 cGy of total body irradiation(TBI) and were reconstituted within 4～6 hours via i.v.injection of MNC from C57b1/6 male mice.3 weeks after transplantation,we detected the Y chromosome specific gene in peripheral blood of recipients NOD mice to determine the engraftment of allogenic bone marrow mononuclear cell.We observed the states of blood glucose,body weight and graft versus host disease(GVHD)in recipients mice.Results Fifteen NOD mice developed type 1 diabetes mellitus.The morbility of DM was 53.57%.The MNC of C57b1/6 male mice engrafted successfully in six diabetic NOD mice,and the transplanted NOD mouse had not any clinical signals of GVHD.4 weeks after transplantation,the symptomes of the diabetic mellitus were not improved in recipients mice.During transplantation,no NOD mice died in the sublethal dose group,while two NOD mice(2/4)died in lethal dose group.Conclusion The mode of MNC transplantation of diabetic NOD mice was established successfully.Sublethal dose was better than lethal dose in diabetic NOD mouse.No obvious symptoms of GVHD could be observed in recipients mice. After MNC transplantation 4 Weeks,the symptomes of diabetic mellitus were not relieved in the recipients NOD mice,it maybe related to total body radiation and the natural procedure of type 1 diabetes mellitus.