Engraftment Growth And Differentiation Of Bone Marrow Mononuclear Cells After Intracoronary Transplanted Into AMI Region At Different Times

Objective: To study the engraftment, growth and differentiation of porcine autologous bone marrow mononuclear cell after being intracoronary transplanted into AMI region at different times (2 hours after AMI and 1 week after AMI).Methods: Bone marrow were aspirated from the ileum and bone marrow mononuclear cells(BM-MNCs) was isolated by Percoll gradient centrifugation .And BM-MNC was prelabeled with red fluorescence cell linker PKH26-GL. A 90-minute left anterior descending artery ligation followed by reperfusion was used to produce myocardial infarction. 8-10×10~7BM-MNCs or culture medium of the same volume were intracoronary infused into infarction related artery at 2 hours or 1 week after AMI. Specimens were harvested 6 weeks after AMI for histological study including HE, PTH, immunochemical stain of factor VIII and Desmin, and transmission electromicroscope study. Result: In the specimens of two intracoronary infusion groups, labeled cells were found only in the infracted area and not in the normal tissue. And newborn cardiac cell were found by HE stain, PTH stain and...