The Study Of Combining Multi-tumor Protein Markers To Identify The Cell Clone Origin Of Multinodular Hepatocellular Carcinoma And It's Clinical Significance

Objective To evaluate the value of identifying the cell clone origin of multinodular hepatocellular carcinoma by detecting five tumor protein markers in hepatoma:P53,Survivin,C-myc,AFP and PCNA,and it's clinical significance.Method Group A(experimental group):multinodular hepatocellular carcinoma 35 cases;Group B(positive control):two pieces of inconsecutive tumor tissues in 29 cases with single nodus hepatocellular carcinoma;Group C(normal control):normal liver tissue 10 cases;Group D:portal vein tumor embolus 8 cases.Immunohistochemical S-P method was used to detect the expressions of P53,Survivin,C-myc,AFP,PCNA protein in various noduses,and statistical analysis was performed.Results The expressions of P53,Survivin,C-myc,AFP,PCNA protein had no differences in experimental group and positive control(p＞0.05).16 of 35 cases were dignosised as multicentric origin and the else were unicentric origin by detecting five proteinum markers,portal vein tumor embolus,tumor size,cirrhosis,Edmondson grade,tumor numbers had differences in the two origin groups(p＜0.05).The recurrence time of multicentric origin cases was longer than that of unicentric origin(p＜0.001). Conclusions 1.The detections of P53,Survivin,C-myc,AFP,PCNA protein can identify the clone origins in multinodular hepatocellular carcinoma;2.It's useful to identify the clone origins in multinodular hepatocellular carcinoma by comparing the following factors:portal vein tumor embolus,tumor size,cirrhosis,pathological classification,tumor numbers;3.The time between operation and recurrence of multinodular hepatocellular carcinoma with multicentric origin was longer than that of those With unicentric origin,so improving preoperative liver performance and striving exairesis was significant.