The Influence Of Intrauterine Hypoxia On The Cerebral White Matter Of The Elderly Offspring Of Rats

[Objects] To study the the influence of intrauterine hypoxia(IH) on the cerebral white matter of the elderly offspring of rats.[Methods] S-D rats intrauterine hypoxia model was established, then their offspring were exposed to high-fat diet(HF) and (or) second hypoxia(SH). Immunohistochemistry was used to detected the expression of myelin basic protein(MBP), neurofilament-H+L(NF-H+L), and glial fibrillary acidic protein(GFAP) in the white matter beside the lateral ventricle of the elderly offspring. Meanwhile Motic pathological image analysis system was used to determine the quantity. Microstructures of the myelin, axon and microvascular in the rat brain were observed by electron microscope. Three-factor two-level analysis of variance was applied to analyze the experimental data.[Results] The main effects of IH, HF and SH all reduced the expression of MBP and NF-H+L, and increased the expression of GFAP in white matter of the elderly offspring of rats(P＜0.05). Interaction existed not only between IH and HF, IH and SH, or HF and SH(P＜0.05) but also among IH, HF and SH (P＜0.05). Moreover, there was a high positive correlation between the expression of MBP and NF-H+L in the white matter beside the lateral ventricle(R~2=0.64, P＜0.01). More demyelination, injured axon, damaged microvasculars, neuronal degeneration and apoptosis were observed by the electron microscope in IN group than in the control group. And these pathological lesions in the three factors crossing group appeared severest.[Conclusions] Intrauterine hypoxia brings harmful effects to cerebral white matter of the elderly offspring of rats. IH increases the risk of demyelination, injured axon and astroglia proliferation which are aggravated by high-fat dietand(or) second hypoxia.