Cellular Immunological Analysis Of Keloid

Cellular Immunological Analysis of KeloidObjection: To study the pathological process of cellular immune response in the keloid tissue.Methods:â… : compare the pathological slice stained by HE between the keloid tissue and the normal skin tissue to find the characteristic change of the keloid.â…¡: recognize the lymphocyte in the keloid tissue by the immunohistochemistry experiment.â…¢abstract the mRNA from the keloid and normal skin frozen tissue, then analysis the product by the RT-PCR and electrophoresis to identify the mechanism of lymphocyte in the keloid.Result: We find that there are plenty of lymphocyte masses away from the blood vessels which are surrounded by the collagen. To recognize the CD4 and CD8 on the surface of T cell, we have done the immunohistochemistry experiments. Among the 10 cases(1 failed), 6 cases have CD8 and 3 have CD4( one of them both has CD4 and CD8) and 1 has no CD4 or CD8. The mRNA from the 15 cases of keloid was detected to affirm the active molecules secreted by lymphocyte. 12 case have granzyme B. 11 have perforin. 6 have granulysin. None has granzyme A. 1 case failed in the mRNA abstraction. Among the 4 normal skin, only one had granzyme B. None have other molecules.Conclusion: In the keloid tissue, the lymphocytes gather away from the blood vessels which are surrounded by the collagen tissue. The gathered lymphocytes consist of T cell and NK cell and most of them are T cell. They can secrete perforin,granzyme and granulysin to cause the lysis and apoptosis of the target cells.