Effects Of Hydrocortisone On The Synthesis Of Melanin In A375 Human Melanoma Cells.

Object: To observe the cell growth, melanogenesis and tyrosinase activity in A375 human melanoma cells after treatment with hydrocortisone of different concentration ;to study the mechanism of hydrocortisone in treating vitiligo.Methods: To treat A375 human melanoma cells in different concentration of hydrocortisone,to observe the growth of A375 human melanoma cells by MTT, to measure the content of melanin by spallation of NaOH, to measure the change of tyrosinase with dioxyphenylalanine oxidation.Results:1)When the concentration of hydrocortisone was between 10-7 g/L and 10-5g/L,it showed an increasing tendency of the amount of A375 human melanoma cells with the concentration of hydrocortisone raised, while the concentration of hydrocortisone was between 10-5 and 10-1g/L, it showed a decreasing tendency of the amount of A375 human melanoma cells with the concentration of hydrocortisone raised, compared with the control, when the concentration of hydrocortisone was at 10-5g/L , the cells proliferated obviously, the volume of the cells accreted; while he concentration of hydrocortisone was at 10-1g/L ,the growth of the cells was obviously inhibited ,the cells atrophied even turned circle, the amount of the cells decreased obviously.2)When the concentration of hydrocortisone was between 10-7and 10-3g/L, it showed an increasing tendency of the content of melanin when the concentration of hydrocortisone increased,when it was 10-3g/L, the content of melanin significantly increased comparing with the control; while the concentration was between 10-3g/L and 10-1g/L, the content of melanin got a tendency of decline with the concentration of hydrocortisone increasing; when it was 10-1g/L, the content of melanin significantly decreased comparing with the control.3)When the concentration of hydrocortisone was between 10-7and 10-3g/L,the activity of tyrosinase of A375 Human Melanoma Cells had an increasing tendency with the concentration of hydrocortisone increased, when it was 10-3g/L, the activity was obviously activated compared with the control; while the concentration was between 10-3g/L and 10-1g/L,the activity of tyrosinase of A375 Human Melanoma Cells showed a tendency of decline with the concentration of hydrocortisone increased, when it was 10-1g/L, the activity of tyrosinase was lowed obviously compared with the control.Conclusion: hydrocortisone of certain concentration may promote the growth of A375 Human Melanoma Cells, activate the activity of tyrosinase, enhance the production of melanin. And it provides an experimental evidence of treating vitiligo patients with hydrocortisone.