The Significance Of The Change Of Levels Of The Activated NF-κB System In Peripheral Blood Leukocytes To Chronic Heart Failure

Objective: To evaluate the activation of transcriptional nuclear factor kappa-B (NF-κB) in peripheral blood leukocytes (PBL) from patients with chronic heart failure (CHF) and its clinical significiance.Methods: We divided objects into two groups, group A: 50 CHF patients (age: 64.7±15.6 years, LVEF: 29.7±7.3%, NYHA class: 3.3±0.8); group B: 30 cardiopathes without CHF(age: 67.0±10.0years, LVEF: 66.5±9.0%). We examined their NF-κB in PBL,LVEF,CRP and 6MWT, periphery oedema was observe, too. The immunocytochemical ocalization was used to examine the NF-κB in PBL. NF-κB activation was expressed by the percentage of PBL nuclei stained positively for c-Rel [NF-κB(+)cell%]. Results: The level of the activated NF-κB system, that's NF-κB(+)cells [%] in three groups is respectively that: 44.4±9.2% in group A; 13.1±5.1% in group B, between the two groups there was notable statistic significance (all p <0.01). NF-κB of patients with CHF after cure according to the cure guide of chronic heart failure was 43.6±9.7%, campared with witch of the group of CHF before receiving cure, there was no statistics difference(p=0.056). There were two main clinical related index of NF-κB activation in PBL from CHF patients: 6-minute walking test (R =-0.568,p <0.001) and NYHA class(R=0.540,p<0.001), LVEF had some pertinence with NF-κB, but had no statistics difference (R=-0.270, p=0.058), in addition, presence of peripheral oedema and its severity had some influence(R=0.161, P=0.263), but the serum C-reactive protein and the age had no pertinence with NF-κB, at last, there was no statistic difference between different foundation heart disease, witch result in CHF. (P=0.970) Conclusions: The NF-κB system is highly overactived in PBL from CHF patients, and the degree of its overactived level has consanguineous relation with 6MWT and NYHA, has little relation with LVEF and CRP, and has complicated relation with peripheral oedema. After receiving the current cure, the descending tendency of NF-κB has been seen. It may prognosticate the degree of the graveness and prognosis of CHF, meanwhile whitch may be a potential target for future therapeutic applications.