Expression Of COX-2, PGE₂ And VEGF In Ectopic And Eutopic Endometrium In With Endometriosis And Its Receptors In Ovarian Carcinoma Ascites And Tissue And Its Meaning

Objective: To investigate the expression of cyclooxygenase-2(COX-2), prostaglandin E₂(PGE₂) and vascular endothelial growth factor(VEGF) and their relationship in patients with endometriosis.Methods: Thirty ectopic endometrium and 24 eutopic endometrium from women with endometriosis were selected as study group. Thirty specimens of endometrium from women without endometriosis were obtained as control group. The expression of the COX-2, PGE₂ and VEGF were measured by immunohistochemical method.Results:1. The expression of COX-2, PGE₂ and VEGF in endometrium from women with study group and control group:(1) COX-2, PGE₂ and VEGF expression were significantly higher in ectopic endometrial glandular epithelial cells from patients with endometriosis than those in eutopic endometrium and control group(P＜0.05, P＜0.01). COX-2 and PGE₂ expression in eutopic endometrial glandular epithelial cells were significantly higher than those in control group (P＜0.05, P＜0.01).(2) COX-2 and PGE₂ expression in ectopic endometrial stromal cells from patients with endometriosis were significantly higher than those in control group(P＜0.01). PGE₂expression was significantly higher in ectopic endometrial stromal cells than eutopic endometrium (P＜0.05). COX-2 expression was significantly higher in eutopic endometrium than control group (P＜0.05).2. The expression of COX-2, PGE₂ and VEGF in endometrium during the proliferative phase and secretory phase.The expression of COX-2, PGE₂ and VEGF in endometrium from ectopic endometrium, eutopic endometrium and control group had not circle changes.(1) COX-2, PGE₂ and VEGF expression in glandular epithelial cells from ectopic endometrium were significantly higher than those of control group during the proliferative phase and secretory phase(P＜0.01). PGE₂ expression was significantly higher in ectopic endometrial stromal cells during the secretory phase than that of control group(P＜0.01).(2) VEGF expression were significantly higher in ectopic endometrial glandular epithelial cells than that in eutopic endometrium during the secretory phase (P＜0.05). PGE₂ expression was significantly higher in ectopic endometrial stromal cells during the secretory phase than that of eutopic endometrium.(3) COX-2, PGE₂ and VEGF expression in glandular epithelial cells from eutopic endometrium were significantly higher than those of control group during the proliferative phase (P＜0.05, P＜0.01). PGE₂ expression in glandular epithelial cells from eutopic endometrium was significantly higher than that of control group during the secretory phase(P＜0.01).3. The expression of COX-2, PGE₂ and VEGF in ectopic endometrium and eutopic endometrium about stages of endometriosis had not different.Conclusion: (1) COX-2, PGE₂ and VEGF expression in ectopic endometrium glandular epithelial cells from patients with endometriosis were significantly higher, and COX-2 and PGE₂ expression in ectopic endometrial stromal cells were significantly higher.(2) COX-2 and PGE₂ expression in eutopic endometrium glandular epithelial cells were significantly higher, and COX-2 expression in eutopic endometrium stromal cells was significantly higher. (3) COX-2, PGE₂ and VEGF may promoting the growth of endometriosis.(4) Biology features of the eutopic endometrium from patients with endometriosis perhaps were different with the normal endometrium.