Change Of Leukotriene E₄ In Urine Of Bronchial Asthma Children And Its Clinical Significance

Objective:To investigate the changes of urine Leukotriene E₄(LTE₄)and blood Eosinophil(Eos) in bronchial asthma children during the stage of exacerbation.persistent and remission,and to explore the association between two markers and their clinical significance.Methods:The levels of urine LTE₄ was measured by enzymelinked immunosorbent assay (ELISA)and blood Eos was measured by blood cell analyzer in the asthma children(n=75) during exacerbation(n=30).persistent(n=15)and remission(n=30)stage,as well as normal children(n=20).The exacerbation group was divided into three groups according to the severity of the asthma:mild asthma group(n=10),moderat asthma group(n=10)and severe asthma group(n=10).Results:1.There were no significant difference in the levels of urine LTE₄ between exacerbation and persistent(q=0.35,P＜0.05);the levels of urine LTE₄ in exacerbation were significantly higher than those in remission and normal control group(q=6.87,7.93,P＜0.05);the levels of urine LTE₄ in persistent were significantly higher than remission and normal control group(q=6.08,7.20,P＜0.05);the levels of urine LTE₄ in remission were higher than normal control group(q=3.86,P＜0.05).2.There were no significant differences in the levels of blood Eos among exacerbation,remission and normal control group(q=1.70,2.96,2.11,P＞0.05). The blood Eos of persistent was significantly higher than exacerbation,remission and persistent normal control group(q=4.32,6.17,8.55,P＜0.05).3.In the exacerbation group,there were significant differences(P＜0.05)in the levels of urine LTE₄ among mild asthma group,moderat asthma group and severe asthma group(q=4.17,9.16,6.53,P＜0.05);the levels of urine LTE₄ increased with the severity of asthma.4.There was positive relation between the levels of urine LTE₄ and blood Eos count in persistent group(r=0.656,P＜0.05);There was no relation between the levels of urine LTE₄ and blood Eos count in exacerbation and remission (r=0.184,0.156,P＞0.05);Conclusion:We found the levels of urine LTE₄ of each stages of asthma children obviously increased compared with normal control,the change of urine LTE₄ was consistent with the severity of asthma,that indicated Leukotriene is contributed to the development of asthma children.The levels of urine LTE₄ may serve as an index of the degree of asthma attack,the measurement of urine LTE₄ was simple.convenient and harmless,It may be a better marker of airway inflammation for asthma childrenand and can be used to judge the severity of bronchial asthma and guide asthma management.