Effects Of Propofol In TNF-α Induced Apoptosis In Endothelial Cells Derived From Human Umbilical Vein

Objective:To investigate the protective effects of propofol in clinical relevant concentration on TNF-αinduced apoptosis in endothelial cells derived from human umbilical vein(HUVECs)Methods:The third and fourth passages of the cultured endothelial cells were divided into 5 groups.0,0,25,50 or 100μmol·L^-1propofol was added to the cultured cells respectively and the cells were incubated for 30 rain.Then TNF-αwas added to the cultured cells(except the control group,the final concentration of TNF-αwas2000 U/ ml)which was incubated for 12h.early apoptosis was detected by using Flow Cytometry(FCM)technique,for Caspase-3 mRNA Semi-quantitative examination using RT-PCR technique.Results:Early apoptosis rate and levels of Caspase-3 mRNA were very high in TNF-αgroup(no propofol was added)as compared with the control group(P<0.05).②The process can be inhibited by pretreatment with propofol(P＜0.05 or P＜0.01).With increasing concentrations of propofol.Early apoptosis rates were significantly decreased in TNF-αinjured cells.③The process can be inhibited by pretreatment with propofol(P＜0.05 or P＜0.01).With increasing concentrations of propofol.Levels of Caspase-3 mRNA were significantly decreased in TNF-αinjured cells.Conclusion:Propofol in clinical relevant concentration has significant protective effects against TNF-αinduced apoptosis in HUVECs.