The Study Of Association Between Apolipoprotein(a) Gene Enhancer Polymorphism And Stroke

Objective Increased plasma concentration of lipoprotein(a) [Lp(a)] represents an established risk factor for stroke. The plasma Lp(a) level more than 90% genetically determined by the apolipoprotein(a) [Apo(a)] gene. To explore the association ofApo(a) gene DHⅢenhancer-1230A/G polymorphism with the occurrence of stroke and the plasma Lp(a) level in han population of Hunan province.Methods The study population was comprised of 150 patients with cerebral infarction, 150 patients with cerebral hemorrhage and 122 healthy individuals. The Apo(a) gene DHⅢenhancer -1230 genotypes were determined by polymerase chain reaction(PCR), digestion by restriction fragment length polymorphism (RFLP) and verification by DNA sequencing. The plasma Lp(a) level was assayed by immunoturbidimetry.Results1. In the study of health han population, The frequency of Apo(a) gene DHⅢenhancer -1230 genotypes are 16.4% (AA), 40.0%(GG), 43.6%(AG), the frequency of A/G altelic is 0.38/0.62. 2. Apo(a) gene DHⅢenhancer -1230 polymorphism revealed significant difference of genotype and allelic distribution in cerebral infarction patients and controls(P=0.006, P=0.01). The GG genotype frequency and T allele frequency in cerebral infarction patients were significantly higher than those in controls. There were no significant difference of genotype and allelic distribution of Apo(a) gene DHⅢenhancer -1230 polymorphism in cerebral hemorrhage patients and controls(p=0.15, p=0.051).3. The plasma Lp(a) level in each genotype of Apo(a) gene DHⅢenhancer -1230 in cerebral infarction patients was higher than that of controls (P＜0.05). In cerebral infarction patients, there were no significant difference observed in Lp(a) level among the three genotypes.ConclusionsThese findings suggest that Apo(a) gene DHⅢenhancer -1230A/G polymorphism might a predisposing risk factor for cerebral infarction determined by G allele, however, they might not association with cerebral hemorrhage in han population of Hunan province.