Study On Infection And Epidemiology Of Methicillin-resistant Staphylococcus Aureus

Objective To acquire the information about the prevalence of MRSA in patient and surrounding.To understand the susceptibility of MRSA to 14 antibiotics. To understand the possible sources and transmission by Homology Analysis in MRSA isolates collected from patient and surrounding.Methods MRSA were identified by their resistance to cefoxitin of disk diffusion and mecA PCR.The K-B method was applied for the drug susceptibility test. RAPD with the optimization condition was used to analysis homology of MRSA isolates.Result The isolated rates of MRSA infection were 58.5% and 38.1% from clinical and surrounding sample respectively.The rates of drug resistance to penicillin,oxacillin, ampicillin,piperacillin/tazobactam, cefotaxime, cefepime, meropenem, ciprofloxacin, erythrocin and tetracyline were 100%.MRSA isolates were highly resistance to amikacin and rifampicin; All MRSA were sensitive to vancomycin and teicoplanin. The numbers of amplified DNA fragments by RAPD were 4 to 6,The main type of the hospital is type I in the five classified genotypes.Conclusion The isolated rate of MRSA was high, Clinical chemotherapy must according to the susceptibility test because of their multi-drug resistance. The dominant epidemic strain of MRSA was type I and clone spread might exist. Extrinsic process was the main source for nosocomial infection caused by MRSA and the hospital must take effective measure to decrease and control nosocomial infection. RAPD is suitable for molecular epidemiology with high powerful discrimination, simplicity and rapidness.