| PART1 The Observation and analysis about Opacity and Pathological Changes of Rabbit Lens in the Early Period after the Operation of Vitrectomy with Nonexpansile Perfluoropropane GasObjective: In this part, we observed the microstructure changes and opacity degree of rabbit lens in the early period after the operation of vitrectomy with nonexpansile perfluoropropane gas, And tried to study the relativity between the microstructure changes, lens opacity and perfluoropropane gasMethods: 42 new zealand health rabbits (84 eyes) were assigned randomly to three groups: normal group, control group and experimental group.The A group-6 rabbits (12 eyes) was normal group; the B group-36 eyes were control group, performed the operation of simple vitrectomy with BSS; the C group-36 eyes were experimental group, performed the operation of vitrectomy with nonexpansile perfluoropropane gas.The intraocular pressures were measured with Schiotz indentation tonometer, and the lens shape were observed with slit lamp microscope on the 1st,3th,8th,35th,45th day after operation.The optical microscope specimens were made with rabbit lens on the 1st,3th,8th,35th,45th day after operation.The electron microscope specimens were made with rabbit lens only on the 45th day after operation.The microstructure changes of LECs, equatorial region and cortex below posterior capsule of rabbit lens were observed with optical microscope and the ultrastructural changes of LEC were observed with transmission electron microscope.Results: 1. IOP: There was no significant difference (P＞0.05) of IOP among the three groups on the 1st,3th,8th,35th,45th day after operation.2. The lens opacity degree: The opacity rate of normal group, control group and experimental group was0.00%, 0.00%, 94.44%.There was significant difference (p＜0.0167) between experimental group and the control group, normal group on the 1st,3th and 8th day after operation. The occurrence rate and degree of cataract was positively correlated with the area and time of contact between perfluoropropane gas and lens, but the opacity alleviated or dead away gradually with the disappearance of perfluoropropane gas.3. The microstructure changes: The rate of lens microstructure irreversible changes of normal group, control group and experimental group was0.00%,0.00%,33.33%, there was significant difference (p＜0.0167) between experimental group and the control group, normal group on the 35th,45th day after operation.4. The ultrastructural changes of LECs: There was no evident abnormal of control group and normal group LECs on 45th day after operation.But the myeloid body was finded in experimental group LECs,the nuclears deformed distinctly and there were a lot of mitochondrias collected around the nuclear, they swelled and vacuolized apparently.Conclusions:1. The perfluoropropane gas was one of the main reasons of the early cataract after the operation of vitrectomy with nonexpansile perfiuoropropane gas.2. The occurrence rate and degree of cataract was positively correlated with the area and time of contact between perfluoropropane gas and lens, but the opacity alleviated or dead away gradually with the disappearance of perfluoropropane gas.3. The perfluoropropane gas could cause the transient cataract in the early period after this operation and the permanent damage of LECs.PART2 The Detection and Analysis of T-SOD and MDA of Rabbit Lens in the Early Period after the Operation of Vitrectomy with Nonexpansile Perfluoropropane GasObjective: In this part, we try to study the relationship between oxidative damage and the early pathological changes of rabbit lens after the operation of vitrectomy with nonexpansile perfluoropropane gas, by the detection of T-SOD activity and MDA content of different ages after operation.Methods: 45 new zealand health rabbits (90 eyes) were assigned randomly to three groups: normal group, control group and experimental group.The A group-15 rabbits (30 eyes) was normal group; the B group-30 eyes were control group, performed the operation of simple vitrectomy with BSS; the C group-30 eyes were experimental group, performed the operation of vitrectomy with nonexpansile perfluoropropane gas. Selecting 6 lens from every group on the 1st,3th,8th,35th,45th day after operation, Making tissue homogenates of them and detecting their T-SOD activity and MDA content by using spectrophotometric method.Results:1. The change of T-SOD activity of the rabbit lens after operation: The T-SOD activity of normal group was hightest, experimental group's was lowest,and there was significant difference (P＜0.01) among three groups in every periods after operation.2. The change of MDA content of the rabbit lens after operation: The MDA content of normal group was lowest,experimental group's was hightest,and there was significant difference (P＜0.01) among three groups in most periods after operation.Conclusions: Oxidative damage mechanism may be involved in the early damge of rabbit lens after the operation of vitrectomy with nonexpansile perfluoropropane gas. |
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