| IntroductionThe temperature of endotherm and human body never exceed a certain limit (human body' s temperature never exceed 41(?)) when they communicated by virus et al. These phenomenon called temperature limit. But the biology mechanisms of fever control of body are not clear. Lipopolysaccharide (LPS) can raise body temperature as exogenous pyrogen. Arginine vasopressin (AVP) can modulate the thermoregulatory response during fever and be referred as endogenous antipyretics, and the VSA of the limbic system is believed to be the specific central site where the antipyretic effects of AVP are mediated. Experimental evidence indicates that AVP when perfused into the VSA of the rabbits can suppress endotoxin fever significantly.Heat shock transcription factor (HSF) are sets of proteins rifeness exist in boilogy cell. Their structure and function have abroad alike, including HSF1(?) HSF2(?) HSF3(?) HSF4．HSF1 is very sensitivity to heat. On the heat stimulative, HSF1 monomer can polymerizate trimer to control the expression of Heat shock protein 70 (HSP70) . Our laboratory' s study results show that HSF1 can depress exterior EP and central fever medium' s production. But whether the polymerization of HSF1 taking part in the control of fever or connecting to the AVP' s negative modulation in the fever are not clear.TO observe the effect of the polymerization of HSF1 on the febrile response and the content of AVP in brain in fever, LPS used to make rabbit fever model and quercetin perfused into the central to suppress the HSF1 activating. This study is further investigating of HSF1 action in thermoregulation and the possible central mechanisms.Material and Methods1．Animal grouping, model preparation and Sample collecting(1) Animal grouping The 70 male healthy New Zealand rabbits were rando-mly divided into 4 groups: the control group (N), the quercetin group (Q), LPS-feverish group (L), the quercetin + LPS-feverish group (Q+L).(2) Surgery Under anesthetized with pentobarbital sodium (3 ml(?)kg i.v.), a 1 mm of diameter connula was implanted into P1L4-5H5 (the lateral cerebral ventricle) by stereotaxic apparatus according to the Sawyer' s rabbit brain altas, the cannula was anchored with dental acrylic. Rabbits were individually housed before experiment.(3) Fever model Intravenous with 0．5 (?)g(?)ml LPS-saline (1 (?)g(?)kg i.v.), All injected materials were dissolved in sterile saline.(4) Sample collecting Take off hypothalamus at the just time of every model. Then put them part hypothalamus into liquid nitrogen 20 min and at last, put them into-70 (?) refrigerator to examine. At the same time treat the other hypothalamus and VSA and put them into-20 (?) refrigerator to examine.2．Measured itemsChange in body temperatue was presented and analyzed with (?)T and TRI; the expression of HSF1 and HSP70 in hypothalamus was used Western blot analysis; the expression of AVP in hypothalamus and VSA use Radialization immunoassay.3．Statistic analysisAll the data use ((?)(?)s) to express. Use SPSS11．0 to analyze. Use Correlation analysis to analyze relativity.Results1．The body temperature change Intravenous injection of LPS (1 (?)g(?)kg) produced double-phase fever in rabbits. The body temperature increases to the first peak value at 60 min ((?)T: 1．32 (?) 0．24 (?)). To the second peak value at 180 min ((?)T: 1．84 (?) 0．35(?)). The sequence of the maximum temperature change ((?)tmax) from low to high :Q |
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