Adenovirus Vector Mediated Rat Insulin-Like Growth Factor 1 Gene Prevents Streptozotocin Induced Cell Impairment On Islet β-Cells

Objective To investigate the protective role of adenovirus vector mediated ratinsulin-like growth factor 1(rIGF-1)gene on streptozotocin-induced isletβ-cellsimpairment.Methods Recombinant adenovirus encoding rIGF-1 gene infect to RINm5F cells.RIGF-1 gene expression in RINm5F cells was detected by RT-PCR method. Thenstreptozotocin was used to induce RINm5F cells impairment. The levels of nitric oxidewere detected in cells culture supematants. The cells function was evaluated byglucose-stimulated insulin production. The apoptosis was analyzed by flow cytometry.Thiaoollyl blue viability assay was applied to exam the number of viable cells.Results The rIGF-1 gene can expresse in the RINm5F cells. RIGF-1 can inhibite isletcell apoptosis and significantly decrease the level of NO induced by streptozotocin, whilesignificantly increase insulin secretion and cells viability.Conclusion Adenovirus vector mediated rIGF-1 gene can expresse in the RINm5F cells.Locally produced IGF-1 from cultured islets may be beneficial in protectingβcellsfunction. And the apoptosis induced by streptozotocin may be NO-dependent.