Experimental Studies Of The Death Recepter And FADD In The Pathogenesis Of Parkinson Disease

Objective: To study the expression of the protein and mRNA of Fas death recepterand FADD in Parkinson'disease (PD) mouse medal at defferent times, and dynaticobserve the expression of Fas and FADD in the PD mouses's substantia nigra andcorpus striatum. Exploring the action of Fas,FADD in the PD from the protein and nucleicacid can more investigate the PD apotosis mechsim, and can help us find themedicine to dopamine neuro apotosis.Method: 72 rats were choosed and individed into control groups and experimentalgroups. Experimental groups include model-three day, model- one week, model-twoweek, model-three week, model-four week and evry group has 12 rats. 6-OHDA wasinjected into substantia nigra with spacial orientation as PD medals, and normal saline ascontrol groups. to inject apomorphine before death and observe ratscircumgyrate. Immunohistochemistry methed hibrization were used to detect the proteinexpression of Fas, FADD and Th, and hybridization in situ used to detect theexpression of FasmRNA and FADDmRNA.Result: The protein expression of Fas,FADD and in PD rats are active comparingthe control groups espetially 2 and 3 week groups. All the defference are significantstatistically in groups (p＜0.01). Fas and FADD have relativity. The mRNA expression ofFas,FADD in PD rats are also active comparing the control groups espetially 2 and 3week groups. All the defference are significant statistically in groups(p＜0.01). FasmRNAand FADDmRNA have relativity. The expression of TH was reverse, the control groupswere obviously active, the medals were lssen. All these indicate Fas FADD participate inthe Pathogenesis of PD. We speculate that the access of apotosis by Fas is one of themechanism of PD.Conclusion: The increase of the protein and mRNA about Fas and FADD and thedecrease of TH, indicate Fas FADD participate in the Pathogenesis of PD. We speculatethat the access of apotosis by Fas is one of the mechanism of PD.