The Study Of TLR2 Gene Expression And The Study Of ASO And IL-8 Levels In Peripheral Blood Of Psoriatic Patients

Objective To detect the expression of toll-like receptor 2 (TLR2) mRNA in peripheral blood monocytes of psoriatic patients and examine the serum level of interleukin-8 (IL-8) and antistreptolysin O(ASO) so as to explore their relevance to psoriasis.Methods The levels of TLR2 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) method; Serum ASO and IL-8 were detected by latex agglutination assay and enzyme linked immunosorbent assay (ELISA).Results 1. The expression of TLR2 mRNA in psoriatic patients was higher than in controls (0.9444±0.1586, 0.7524±0.1343, t=5.5407, P＜0.01); There was no significant difference between guttate and plaque psoriatic patients (0.9502±0.1823, 0.9356±0.1183, t=0.3178, P＞0.05). 2. the positive rate of ASO in psoriatic patients was higher than in controls (x~2=8.6044, P＜0.01); And the ASO positive rate in guttate patients was higher than in plaque psoriatic patients(x~2=9.7801, P＜0.01). 3. The level of IL-8 in psoriatic patients was higher than controls (82.200±8.2722, 26.2667±8.8957, t=28.4627, P＜0.01); There was no significant difference between guttate and plaque psoriatic patients (82.8333±9.2086, 81.2500±6.7424, t=0.6592, P＞0.05). 4. The expression of TLR2 mRNA in psoriatic patients with positive ASO was higher than that in patients with negetive ASO (1.0197±0.1523, 0.8709±0.1863, t=2.4066, P＜0.05); The expressions of TLR2 mRNA and IL-8 in psoriatic patients had positive correlation (r=0.6369, P＜0.01).Conclusion Our findings highlight the possibility that TLR2 may be involved in the initiation and development of psoriasis by recognizing the invading streptosis and the following generation of cytokines.