| Objective: To investigate the method of extraction and determination of Baicaleinand Baicalin in S. baicalensis Georgi and S, amoena. To establish theHPLC-fingerprints of S. baicalensis Georgi and S. amoena.Details of the study: 1. Extraction method of S. amoena and S. baicalensis Georgi. 2.To study on the determination of Baicalein and Baicalin by high performance liquidchromatography. 3. To study on HPLC-fingerprints of S. baicalensis Georgi andS.amoenaMethods: To study on extraction method of the major effective ingredients inS.amoena and S. baicalensis Georgi, to determinate the Baicalein and Baicalin byRP-HPLC at the same time, to establish the HPLC-fingerprints of S. baicalensisGeorgi and S. amoena.Results: The optimal parameters were 70％ethanol , 2. 5hours and 1:150 materials(g/mL). The Baicalein and Baicalin were separated on a ZORBAX Stable Bound(150mm x4.6mm, i.d. 5.0μm) chromatographic column, with methanol -water(contained 1.0％methanoic acid) as mobile phase at a flow rate of 1.0mL/min. Thecolumn temperature was controlled at 30℃and the detection wavelength was at274nm. Comparative. study on HPLC-Fingerprints of S.baicalensis Georgi andS.amoena. Zorbax C18 (250mm×4.6mm i.d., 5.0μm) column was used. The mobilephase was acetonitrile-water (contained 1.0％methanoic acid) for gradient elution.Samples of different S.amoena had high similarity, but samples of S.amoena and S.baicalensis Georgi had difference in fingerprint graphics. The average similarity for S.amoena and S. baicalensis Georgi was 0.859.Conclusions: The optimal extraction method of Baicalein and Baicalin was gained byexperiment, which was accurate and stable; The RP-HPLC fingerprint method isrepeatable and feasible in identification of S. baicalensis Georgi and S. amoena. |
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