Alteration Of Cellular Immune Function Of Rats With Implanted Breast Cancer After Radiofrequency Ablation

Objective: To study the changes of cellular immune function in rats with implantedireast cancer after radiofrequency ablation ( RFA) . This study was des igned toevaluate the impact of RFA on splenocyte immunity of rats bearing Walker-256Breast cancer through detecting before and after theapy.Method: A total of 32 SD rats were randomized into 4 groups : RF, surgical resection,tumor-bearing control, and normal control groups . The contents of T cell phenotypein blood was detected with flow cytometry (FCM) and the contents of solubleinterleukin-2 receptor (sIL-2R) was measured by enzyme -linked immunoabsorbentas say (ELISA) before and after RFA 1～5 weeks.Results: Before RFA, CD3~+,CD4~+,CD8~+ T cell, CD4~+ / CD8~+ and sIL-2R in the groupof tumor-bearing control rats were significantly different than those in the normalcontrol groups. After RFA, CD3~+,CD4~+,CD4~+ / CD8~+ in the RF rats model increasedsignificantly ( P < 0. 05) compared with the group of tumor-bearing control, it showedsignificantly different ( P < 0. 05) .and CD8~+ T cell,expression of s sIL-2Rdecreased. It also showed significantly different ( P < 0. 05) . the RF rats showedsignificantly different than the group of surgical resection after 1,2,3 weeks ( P < 0.05).Conclusion: RFA can efficiently stimulate splenocyte activation and proliferation intumor-bearing mice , and enhance splenocyte cytotoxicity to tumor cells . RFA mayactivate and improve cell-mediate immune function to some extent.