Compariative Research Of The Pharmaceutic And Pharmacodynamics Among Different Breed Bulbus Fritillaria Thunbergii

Bulbus Fritillaria Thunbergii is the dried bulbs from perennial herb of Liliaceae. Cold in property, bitter in flavour; meridians entered heart and lung. It can refrigeration and eliminating stagnation, anti-cough and disperse phlegm. Peimine and peiminime are main component, have manyways Pharmacologic Effect.The text has carried on detailed analysis to these respects:1 Character identification and micro-characteristics of Bulbus Fritillaria Medicinal Materials. At the same time, peimine and peiminine were identified by TLC, the results were in line with the requirement of Chinese Pharmacopeia 2005 version.2 The orthogonal design was used, survey the extraction solvent, extraction time, consumption of ammonia liquor , infiltration time of ammonia liquor, to select the optimal extract technic, the best result is: ammonia liquor is 2 times, infiltration 15 minutes, chloroform is 2 times as much as raw materials, accede to soxtherm distil and decocting 4 hours.3 Determination of peimine and peiminine by HPLC, according the result choose the Bulbus Fritillaria Thunbergii to do Pharmacodynamics comparae. Result: harvest at the first and second ten-day period of April from Yinxian, Zhangcun and Jinhua.4 With the method of ammonia caused mice cough, determination of the cough incubation period and degree, survey the effect of anti-cough of the Bulbus Fritillaria Thunbergii which harvest at the first and second ten-day period of April from Yin(washing; clam power), Zhangcun and Jihua. Result: the cough incubation period, the low doses groups were no difference; the middle doses groups Yin(clam power) > Jinhua > Yin(washing) > Zhangcun; the high doses groups Yin(clam power)> Jinhua>Zhangcun> Yin(washing); the cough degree, there was no difference.5 With the method of the excretion quantity of phenofulphthalein in trachea of mice, determination of phenol red output of trachea in mice ,suvrey the effect of disperse phlegm of the Bulbus Fritillaria Thunberigii which harvest at the first and second ten-day period of April from Yin (washing; clam power), Zhangcun and Jinhua. Result: no obvioμs difference between medicines groups and contrast group (p>0.05)。6 With the method of inject 2,4-dinitrophenol in back caused rat heating, determination the rat anus temperature per 20 minutes, draw the change plot of the rat anus temperature in 3 hours,survey the effect of refrigeration of the Bulbus Fritillaria Thunbergii which harvest at the first and second ten-day period of April from Yin(washing; clam power), Zhangcun and Jinhua. Result: the change of the rat anus temperature was slowly in Jinhua group, Jinhua group can effectively inhibit the fever, have good effect.