Relationship Between Polymorphism Of GNAS1 And Genetic Susceptibility To Bladder Cancer

Purpose: To investigate the relationship between polymorphism of GNAS1 and genetic susceptibility to bladder cancer.Materials and methods: The study was a case-control study, which included 47 case of bladder cancer and 56 age-sex-matched population controls during March 2006 to August 2006. All case samples come from People's Hospital of Gansu Province and The Firth, Second Affiliated Hospital of Lanzhou University. 4ml blood samples was collected in EDTA anticoagulant tube and restored in -20°C icebox. The case group included 40 men and 7 women, mean age was 62.1±19.4 years, smoker and nonsmoker were 41 and 6, respectively. All patients were TCC confirmed by biopsy followed cystoscopy or surgery cystectomy . The tumors were graded and staged by standard histologic analysis . The controls were confirmed health including 46 men and 10 women, mean ages was 60.4±20.1 years, smoker and nonsmoker were 37 and 19, respectively. We detected the genotype of all population through PCR-RFLP and analyzed by SPSS10.0. The statistical difference was p<0.05.Results: The frequencies of genotype TT in controls(55.4%) were significant higher than in cases(17.0%), in contrast, genotype CC in cases (33.6%) were higher than controls(16.1%), there were significant statistic in two groups(p<0.05). As well as in alleles T and C, the frequencies of alleles T in cases (32.8%) were lower than controls (67.2%), but the alleles C in cases (62.2%) were higher than controls (37.8%). In cell grade of cases, G1 differed from G3 on genotype frequencies (p=0.038). Also, there was a significant relationship between genotype and pathologic stage.For smoke, the frequencies of genotype TT in cases (7.5%) were lower than controls(56.8%), but genotype CC in cases (50.0%) were higher than controls(25.9%), there were significant statistic between two groups (p<0.05). Multivariate (including ages, agenda, smoking status and genotype) analysis using logistic regression model revealed that only the genotype have statistically significant effect on bladder cancer incidence (P=0.014).Conclusion: The genetic polymorphism of GNAS1 associated with the bladder cancer. The persons carried allele C have high risk to develop bladder cancer, and have advanced cell grade and pathological stage. In contrast, the persons with allele T were different from the persons with C, in our study the allele T was a defensive factor. For smoker, they have a trend to develop bladder cancer because their allele T were fewer compare to nonsmoker's.