Effect Of Matrine On The Expression Of P38MAPK And JNK Of Human Fetal Hepatocytes Preserved From Cryopreservation

Objective: The different concentration matrine was added to human fetal hepatocytes from cryopreservation. By detecting p38 MAPK and JNK, to find protection mechanism of the human fetal hepatocytes on molecule level. Just to find way to preserve human fetal hepatocytes from cryopreservation effectively, and to establish platform for biological reactor, hepatocyte library and transplantation of hepatocyte.Methods: Human fetal hepatocytes were stored from cryopreservation in five different matrine concentration, with a normal cryopreservation as control. Expression of p38 MAPK and JNK in human fetal hepatocytes before and after cryopreservation were measured by Western-Blot.Results: The human fetal hepatocytes preserved in different cryoprotectants showed different livability, adhesion, morphological manifestation and cell function, and in 8mg/L matrine was the most obvious. Expressions of p38 MAPK and JNK after thawing were higher than before thawing, but lower when added with Matrine. It hints that cryopreservation prompts the expression of p38MAPK and JNK in human fetal hepatocytes, and matrine inhibits the activation of conduction pathway.Conclusions: Matrine at some concertration has a well protective effect on the hepatocytes and 8mg/L was the best. But the effect depend on the concertration of matrine. Inhibiting beginns when matrine increasing to 64mg/L. Maybe by inhibiting activation of JNK & p38MAPK, matrine protects human fetal hepatocytes when infiltration pressure changed.