Effects Of Modified Kaixin Powder On Behavior And Acetylcholine Esterase MRNA In Vascular Dementia Rat

Objective: Kaixin Powder is an effective formula to treat morbid forgetfulness. Previousexperiments indicate it could degrade blood viscidity, improve microcirculation and cureAlzheimer's Disease, so we deduce that it could prevent and cure Vascular Dementia. Studies onthe therapeutic effect of modified Kaixin Powders on Vascular Dementia rats and the effect ofmodified Kaixln Powder on acetylcholine esterase mRNA expression in hippocampus inVascular Dementia Rat supply the experiment foundation of Kaixin Powder and combinationTraditional Chinese Medicine and Westen Medicine on preventing and treating VascularDementia.Methods: The rat model of Vascular Dementia was established by ligating both commoncarotid arteries with silk thread. After 30-day-treatment, their learning and memory were testedwith Morris maze; 38 days later mRNA expression of AChE was measured with RT-PCRhalf-fix-quantify method; All the data was analyzed by SPSS 13.0.Results:1. The first day's spatial navigation task latencies, 7 days' spatial navigation task latencies,the results of probe task, and acetylcholine esterase mRNA expression in hippocampus of modelcontrol group rats were different from those of blank control group, P＜0.05;2. Compared with model control group, combination of modified Kaixin Powder andTacrine group spatial navigation task latencies were shOrter, in probe task the swimming time,journey ratios and the times through platform zone in the target quadrant were bigger, P＜0.05;3. Compared with model control group, Tacrine group spatial navigation task latencies wereshorter, the acetylcholine esterase mRNA expression was down-regulated, P＜0.05;4. Compared with model control group, Kaixin Powder group and Ramulus Uncariae CumUncis group the swimming time and journey ratios were bigger in probe task, P＜0.05;5. There was no significant difference between combination of modified Kaixin Powderand Tacrine group,Kaixin Powder group,Ramulus Uncariae Cum Uncis group and Tacrinegroup in spatial navigation task latencies, the swimming time, journey ratios and the times through platform zone in the target quadrant in probe task, P＞0.05;6. There was no significant difference between combination of modified Kaixin Powderand Tacrine group,Kaixin Powder group and Tacrine group in acetylcholine esterase mRNAexpression, P＞0.05.Conclusions:1. Two-vessel occlusion of the common carotid arteries maybe up-regulateglyceraldehyde-3-phosphate dehydrogenase mRNA expression in hippocampus of VascularDementia Rat;2. Modified Kaixin Powder, modified Kaixin Powder with Ramulus Uncariae Cum Unciscould be good at enhancing VD rats' memory; Tacrine could be good at enhancing VD rats'learning; In a woed, modified Kaixin Powder, modified Kaixin Powder with Ramulus UncariaeCum Uncis could enhance VD rats' learning and memory as well as Tacrine. We could use one ofthem acording to clinical need;3. Combination of modified Kaixin Powder and Tacrine could enhance VD rats' learningand memory better than modified Kaixin Powder or Tacrine;4. Tacrine could down-regulate acetylcholine esterase mRNA expression in hippocampus ofVascular Dementia Rats, modified Kaixin Powder, combination of modified Kaixin Powderand Tacrine down-regulate acetylcholine esterase mRNA expression as well as Tacrine.