The Ragulatory Effect Of Somatostatin On The Growth Of Gastric Carcinoma Cell Line

Gastric carcinoma is one of the most frequent malignant cancers,The morbidity and mortality occupy the first place of the digestive tract tumor.Gastrectomy is the chief mothed to treat advanced carcimoma of stomatch at present,but the effect of chemothapy and the survival ratio is low,to seek more treat target so as to raise the treat level of gastric carcinoma has become the hot spot of clinical research.In recent years,the anti-tumor role of somatostatin and its analogs is increasingly paid close attention to.Researchs have discovered that somatostatin has the anti-tumor role to many tumor and nomal tissue.The experiment aim at to explore the extraneous inhibitory action of somatostatin analogue on gastric carcinoma cell line.Objectives To study the regulatory effect of somatostatin analogue octreotide on the growth of gastric carcinoma cell line SGC-7901 and to approach the possible mechanism.Methods Treat the moderately differentiated human gastric carcinoma cell line SGC-7901 cultivated in vitro with octreotide,Use 5-FU as masculine medicine contrast,use the human fibroblasts as normal cell contract.Observe the inhibitory action of octreotide on gastric carcinoma cell and the human fibroblasts through MTT assay,and compare the action with 5-FU;Observe apoptosis through the fluorescent microscope;Analyze the influerce of octreotide on the cellcycle and the rate of apoptosis of gastric carcinoma cell; Determine the change of IGF-1 content in cell culture fluid by radio-immunity method.Results Octretide could inhibitit the growth of gastric carcinoma cell from the concretration of 100ug/L to 2mg/L,At low concretration(50ug/1)the ettect is inevident, compared with the control group P>0.05.Octretide have no evident inhibitory effect on human fibroblasts,because of the inhibitory effect of each group have no evident difference(P>0.05);there is no difference between octretide(500ug/L)and 5-FU(50mg/L) in their inhibition on SGC-7901(P>0.05);After treated with Octretide(500ug/L)for 48h,the morphlogy change of apoptosis could be observed through fluorescent microscope;treated with Octretide(500ug/L)for 48h,Most cells were blocked at G0/G1 phase(72.07±2.40), cells at S phase decreased signiticantly(14.99±1.42),the proliferation of cell was inhibited obviously,the rate of apoptosis increased(21.40±2.71);After treated with different concertration of octretide,the content of IGF-1 in cell culture fluid was low significantly than control group(P<0.01).Conclusion 1,Octroetide can inhibit the growth of gastric carcinoma cell in vitro.2,Octroetide can produce effect of inhibition on target cell only.3,Octroetide can inhibit the prolifetation directly through the induction of blockage at G0/G1 phase and apoptosis.4,Octroetide inhibit the growth of cell indirectly by inhibiting the secretion of growth factors such as IGF-1.