| Background There are many oncogenes, anti-oncogenes, genesthat repair DNA and related protein molecule in the development ofhepatocellular carcinoma (HCC). The DNA polymerase is very importantin the mechanism that keep the integrity of genome in the most cells. It isthought that DNA polymeraseβ(DNApolyβ) mainly supply the gaps ofmono-ribonucleotide in the course of base excision repair (BER). Themutation of DNApolyβgene may produce the abnormal DNApolyβandimpair the function of BER in cell. In the end, it will influence thestability of the chromosome in the cell and increase the incidence ofproducing tumor At the present time , the concrete mechanism ofDNApolyβis not identified in the tumors that come from differenttissues. Ferritin (FT) is the storage form of iron in cell. The iron is veryimportant in the course of the DNA synthesis, electrontransferring, oxygen transporting and so on A number of iron arerequired in the growth and generation of tumor. There are different expression of FT in the different tumor.It is not identified how the FTwork especially in the HCC. The research is done on the expression andits relationship with clinicopathological feature of DNApolyβand FT inHCC.Objective TO study the expression and its relationship with theclinicopathological feature ofDNA polymeraseβand FT in HCCMethod It is selected that the 67 fresh samples of HCC surgicallydissected and 16 normal hepatic tissue. Then,each tissue slice is dyedwith the polyclonal antibody of DNApolyβand FT through the EnVisionTM immunohistochemical method. It is with the X~2 test, t test and ranktest that the research is done on the expression and its relationship withclinicopathological feature of DNApolyβand FT in HCCResult 1. It is 59.70 percent (40/67) that the positive rate ofexpression of DNApolyβin the HCC, which is higher than the 12.5percent (2/16) of normal hepatic tissue (p＜0.01).And, It is 35.82percent(24/67) that the positive rate of expression of DNApolyβin the HCC,which is lower than the 81.25 percent (13/16) of normal hepatic tissue(p＜0.01). 2. The score of expression of DNApolyβis increased with thedecreasement of the extent of the cell differentiation in the HCC. Itsaverage rank is 25.33,28.98 and 40.92(p＜0.05) from the high to lowaccording to the the extent of the cell differentiation in the HCC. Itsaverage rank is 23.88 in the HCC with the negative expression of type B hepatitis while Its average rank is 36.21 in the HCC with the positiveexpression of type B hepatitis. There are the statistical significance in thedifference between the both (P＜0.05).3. The score of expression of FTis decreased with the increasement of the extent of the cell differentiationin the HCC. Its average rank is 47.83, 36.48 and28.67(P＜0.05)from thelow to high according to the extent of the cell differentiation in the HCC.Conclusion 1. The expression of DNApolβin HCC issignificantly high.It indicates that the expression level of DNApolβhasrelation with the aggravation of HCC. It is an important biologicalparameter that shows the progress and prognosis of HCC. Moreover, theexpression level of DNApolβin the HCC with the positive type Bhepatitis is higher than it in the HCC with the negative type B hepatitis. Itindicates that hepatitis B virus impair the biological function of DNApolβand increase the incidence of hepatic mutation. 2. The expression of FTin HCC is significantly low. But, the expression level of FT in thewell-differentiated HCC is higher than it in the poorly differentiated HCC.It indicates that the expression level of FT has relation with theaggravation of HCC and promotes the occurrence and development ofHCC.
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