Study On Poly L-lactic Acid Vascular Anastomotic Cuff

Objectives: This study was aimed to evaluate the biocompatibility and biodegradation of self-designed PLLA vascular anastomotic cuff, and the preliminary vascular anastomotic experiments were performed on animals with PLLA cuff.Methods: 1. PLLA vascular anastomotic cuffs were made by molding; 2. The biocompatibility of PLLA was evaluated both in vitro and in vivo including cell toxicity test, acute and subchronic systemic toxicity test, hemolysis test, hemopexis test, micronucleus test and intraabdominal implantation test; 3. The biodegradation was studied by degradable test both in vitro and in vivo, serum lactic acid index; 4. Vascular anastomotic experiments were performed on animals with PLLA cuff.Results: There was no obvious change of cells proliferation after L929 mouse fibroblasts had been treated by extraction of PLLA. The cytotoxicity stage of all levels of extraction was below 1, measured by A value of MTT assay; The extraction of PLLA didn't have acute and subchronic systemic toxicity to animals; There wasn't mal-response in hemolysis test (the hemolytic rate in vitro was 1.99%); PLLA didn't cause hemopexis in vitro; The medullary micronucleus frequency of PLLA was 1.0±0.8‰, being less than 5‰; The responses of tissue were light after implantation in all periods; PLLA could be degraded in vitro and in vivo, there were no significant differences on the serum lactic acid index change of rat between pre-implanting and post-implanting(P＞0.05). The infrahepatic venae cava of rats were anastomosed with PLLA cuff, the results showed it could reduce time of anastomosis and prevent stenosis of anastomotic stoma to a certain extent.Conclusions: PLLA had no cell toxicity in vitro, no acute and subchronic systemic toxicity to animals; it didn't cause hemolysis and hemopexis; it had also no mutagenic action; its histocompatibility was good. Consequently, the biocompatibility of PLLA was good. PLLA was a degradable biomaterial in vitro and in vivo, and degradation products had no bad influence on organism. Vascular anastomosis could be performed quickly with PLLA cuff, which was reliable.