Kang Xian Fu Fang Ⅰ Inhibited The Expression Of TNF-α And PAI-1 In Alcohol-Induced Liver Disease In Rat

ObjectiveThe occurrence of Alcoholic Liver Disease(ALD)is related with many factors. These are genetic factor, immune factor, oxidative stress.Among these:more and more studies focus are the role of cytokines in alcoholic liver disease. Cytokines take part in the inflammation of liver cell and induce hepatocyte apoptosis. Tumor necrosis factor-α(TNF-α) and plasminogen activator inhibitor type 1 (PAI-1) plays important role in the progress of alcoholic liver disease. In this study we try to demonstrate the dynamic varity of TNF-αand PAI-1 in alcoholic liver disease animal model. In the same time, the affect of KXI on TNF-αand PAI-1 in ALD was also observed. We found that liver damage is related with alcohol dosage. All the results will afford help to the mechanism, prevation and clinical treatment of ALD.Methods1.Alcohol-induced Hepatic Disease ModelRats were divided into three groups randomly. Control group(21 rats) were received normal sodium(2.0ml) instead of ethanol trice a day for 12 w.Alcohol-fed group(21 rats) were infused ethanol intragastricly trice a day for 12w.0-4w, 400ml/L ethanol 8g/kg/d;5-8w,500ml/L ethanol 9g/kg/d;9-12w, 500ml/L 10g/kg/d;Medicine-treated group (21 rats) were admission KXI(4ml/kg/d) twice a day plus equal ethanol with ethanol-fed rats for 12w.2.Histopathological Analysis Rats were killed at the end of 4w, 8w, 12w.A small sample of liver was fixed in formation. Hematoxylin-eosin strain was used for light microscope.3.TNF-αexpressionThe expression of TNF-αwas detected by immunohistochemistry. Paraffin hepatic sections were strained with SABC methods. The antibody was diluted in 1:100.For evaluation the expression, the positive area was detected by Image Analysis.4.PAI-1 expressionThe expression of PAI-was detected by immunohistochemistry. Paraffin hepatic sections were strained with SABC methods.The antibody was diluted in 1:100.For evaluation the expression, the positive area was detected by Image Analysis.5.Statistical AnalysisDatabase was set up in SPSS for windows 11.5. T-test analysis was used to solve the data.Result1. HistopathologyIn ethanol-fed rats, diffuse bubble steatosis and spotty hepatocellar necrosis were observed after 8 weeks. Up to 12 weeks,badly hepatic fatty changes, marked necrosis. In contrast, these alterations were attenuated by KXI administration although mild fatty changes remained.2.The expression of TNF-αThe positive areas of TNF-αlocated in the cell plasm of central vein circumference. TNF-αwas basic no expression in control group.The expression in alcohol-fed group was marked than KXI-treat group.Semi quantity analysis showed TNF-αwas significantly increase with the time of ethanol exposure.3. The expression of PAI-1The positive areas of PAI-1 located in the cell plasm of central vein circumference. PAI-1 was basic no expression in control group.The expression in alcohol-fed group was marked than KXI-treat group.Semi quantity analysis showed PAI-1 was significantly increase with the time of ethanol exposure.Conclusion1.Positive areas located cell plasm of central vein circumference. In the alcohol-fed rats, TNF-α, PAI-1 expression increased with the development of ALD.2.KXI was contributed to inhibit the expression of TNF-αand PAI-1 in alchol-indused liver disease in rats.