| ObjectivePeroxisome proliferation activated receptory(PPARγ) belongs to nucleus hormone receptor superfamily new member. PPARγis an ligand activated transcription factor,it binds with specific DNA response element, then controls gene express. Maim function of PPARγhas been proved that it plays an important role of the messenger in the regulation of the cell differentiation,the cell proliferation the tumour invasion and metastasis. It is short comparatively in studying in gestational trophoblastic disease. This study was conducted to investigate the expression of PPARγprotein in gestational trophoblastic disease as well as to elucidate its significance.Materials and Methods1. MaterialsParaffin-embedded tissues from 60 patients with hydatidiform mole,12 patients with malignant mole,11 patients with trophoblastoma and from 30 case of normal placenta tissues from patients who received artificial abortion at the affiliated hospital of China Medical University between 1988-2006 were analyzed for PPARγprotein expression by immunohistochemical technique, patients with hydatidiform mole with a mean age of 27(27.14±8.03). patients with malignant mole with a mean age of 30 (30.23±8.87). patients with trophoblastoma with a mean age of 34 (34.46±9.44). patients with artificial abortion with a mean age of 26 (26.97±5.13).They had not received either chemotherapy or radiation therapy before surgery and agreed with this experiment. 2. MethodsAll specimens were fixed in 4% buffered formaldehyle and prossed into routinely paraffin-embedded blocks. For each case, four-micrometer sections from these blocks were mounted on silane-coated slides, air-dried. Sections were dewatered and hydrated, and then treated in 3% hydrogen peroxide for 10 min to quench endoengous peroxidase. Prior to staining with the anti-PPARγantibodies, microwave antigen-retrienval technology was used, and then non-immuno animal serum was droped to seal. All procedures were performed at room temperature and in a moist chamker, with a PBS wash after each step. PPARγantibody were droped on the slides. After over night at 4℃, the sections were incubated with them, then after the flushing the slice again, the sections were added a drop of living creature second antibody, after the room temperature fosters 30 minutes, PBS flushes, the DAB coloration, the haematoxylin counterstain, the conventional dehydration, the transparent gum seals solidly.Replaces with PBS first anti-makes the negative comparison, makes the positive comparison with the known positive specimen.3. Statistical methodsStatistical analyses of PPARγprotein expression was carried out by SSPS10.0. Differences were considered significant when P values were less than 0.05.Results1. The expression of PPARγprotein in the four kind of tissue was mostly located at cell nuclei.In normal first trimester tissues, PPARγprotein showed high expression, it was mainiy localized in the nuclei of the villous cytotrophoblast cells, Extravillous cytotrophoblast of cell island and cell columns also showed nuclear PPARγimmunostaining.A striking result was the altered expression patterns of PPARγin pathological tissues.In hydatidiform mole, it was found that It's positive expression, PPARγwas mainly localized in the nuclei of the trophoblastic collections of the pathological villi and in the extravillous trophoblastic cells, the positive cell distributed dispersively or locally. In malignant moles,PPARγshowed a reduced immunostaining, whereas in the choriocarcinoma,only a few trophoblastic cells showed weak PPARγnuclear immunostaining or even without staining. 2. The positive expression of PPARγprotein in hydatidiform moles is 78.57% in high degree hyperplasia, it was 100% in low degree hyperplasia. There is significantly difference between them(P＜0.05). The expression of PPARγprotein in moderate degree hyperplasia of hydatidiform moles is between in high degree hyperplasia and in low degree hyperplasia.Conclusion1. The expression of PPARγprotein was mostly located in the cell nuclei, The PPARγprotein In normal first trimester tissues and In hydatidiform moles showed high expression, but the expression of PPARγprotein decreased or loss significantly in the choriocarcinoma. The expression of PPARγis between the hydatidiform moles and the choriocarcinoma in malignant moles.2. The expression of PPARγprotein is distinguish among different hyperplasia degree of trophocyte cells (P＜0.05),the higher was the hyperplasia of hydatidiform moles,the weaker would be the expression of PPARγprotein.
|
PDF Full Text Request