Simultaneons Determination Of Three Nitrofurans In Animal Feeds By Ultra Performance Liquid Chromatography

Nitrofurans man-made are nitrofurazone , nitrofurantoin and furazolidone mainly. Foods from animals could be secured by determination of nitrofurans in animal feeds. We discussed the conditions of the seperation,extraction and the extracts purified of nitrofurans . A ultra performance liquid chromatography (UPLC)method was established for simultaneous determination of three nitrofurans, nitrofurazone , nitrofurantoin and furazolidone in animal feeds. Nitrofurans in animal feeds were extracted by acetonitrile under the microwave, and determined by ultra performance liquid chromatography with a photodiode-array detector. Nitrofurans were extracted from animal feeds with the microwave at 80℃, 10min,2atm and the extracts were purified by the OasisTM MCX solid phase extract column . The mobile phase considered as an optimum was acetonitrile–glacial aceticacid(0.3%)(10:90,v/v), and the flow was 0.4ml/min . The detector was set at 365nm. The overall average recovery in nitrofuran fortified feeds was 84.5～92.3% for nitrofurazone, 80.0～92.3% for nitrofurantoin, 83.8～92.3% for furazolidone. The UPLC limit of detection, based on a S/N of 3, was estimated to be 0.01,0.015,0.01μg/g for nitrofurazone, nitrofurantoin and furazolidone, respectively. UPLC has showed itself to be a sensitive, selective and rapid method and was successfully used for confirmation analysis of nitrofurazone, nitrofurantoin and furazolidone in animal feeds.