Study On Biotransformation Of Piceid And The Stability Of It's Product

biosynthesis has gained increasing interest during recent years for its exquisite selectivity, high efficiency, friendly environment and can synthesize the compounds which can't be in chemical method .As one of the most important biocatalyst, hydrolases has the significance in biotransformation. Up to now, there are some successful application of hydrolases in asymmetric synthesis and chiral resolution. Meanwhile,it is important significance to useβ-glucosidase which can make some natural products producing new derivates.Among 20 Fungi tested, 8 were screened out which can transform arbutin to a single product by TLC analysis. The product was prepared by the scale-up fermentation, and identified as dihydroxyhenzene by analysis .Then use the 8 Fungi to treat giant knotweed rhizome respectively, the Syncephastrum racemosum 3.264 was found to transform piceid to resveratrol efficenly,the result was detected by TLC and HPLC and identified by ~1HNMR and MS.biotransformations of piceidwere performed by growing culture and resting culture of 3.264 , growing culture was selected to transform piceid.The different substrate concentrations and time-curve of transformation by 3.264 have been tested The hydrolases activity was determined by using TLC and HPLC. the results suggested that the piceid hydrolases was an intracellular soluble protein in cytoplasm. The enzyme displayed the maximum activity at 40～50°C, in pH 4.5～6.5.Resveratrol was separated and purified from giant knotweed rhizome fermented , and it will be oxidized when fermenting.Then the stability of heat and illumination of resveratrol were tested.