Effects Of Soluble Interleukin-1 Receptor TypeⅡ On IL-1α Induced IL-8 And RANTES Expression In Eutopic Endometrial Stromal Cells Of Endometriosis

Objective:There are alterations in both cellular and humoral immunity inwomen with endometriosis (EMs). The complicated cytokine networkconstructed by different cytokines may be involved in thepathophysiology of EMs. Interleukin-l(IL-1)family, IL-8, Regulatedupon Activation, Normal T Cell Expressed and Secreted (RANTES) playimportant roles in the network. Our study emphasis on investigating theeffect of soluble interleukin-1 receptor typeⅡ(sIL-1RⅡ) on IL-1α-induced expressions of IL-8 and RANTES in eutopic endometrialstromal cells (ESC) from patients with EMs and further identify the roleof IL-1 family in immune factor network to provide rational andexperimental evidence for original clinical strategy of endometriosis.Methods:Eutopic ESC from patients with EMs were isolated and cultured invitro. Stromal cells were treated with different concentrations of IL-1α(0,0.1, 1.0, 10, 100 ng/mL) respectively for 24 hours. RT-PCR andEnzyme-linked immuno-assay (ELISA) were used to examine the expressions of IL-8 and RANTES in transcriptional and translationallevels. The concentration of IL-αwhich could remarkably enhance theexpressions of IL-8 and RANTES was used to stimulate ESC for differenttimes. The levels of IL-8 and RANTES were measured at each time pointby RT-PCR and ELISA. Meanwhile, the effects of 1.0μg/ml sIL-1RⅡonIL-1α-induced expression of IL-8 and RANTES were detected.Results:ESC could slightly express IL-8 mRNA and protein as well asRANTES mRNA without stimulation. The levels of IL-8 mRNA andprotein as well as RANTES mRNA were all remarkably increased afterIL-1αstimulation in a dose- and time- dependent manner. IL-8 andRANTES expressions induced by low concentrations of IL-1αwerepartially inhibited by 1.0μg/ml sIL-1RⅡ.Conclusion:IL-1αcan induce IL-8 mRNA and protein as well as RANTESmRNA expression in eutopic ESC of EMs in a dose- and time- dependentmanner. IL-1αlocates upstream in the complicated cytokine network. Itmay contribute to the pathogenesis and development of EMs directly orindirectly, sIL-1RⅡcan partially antagonize the effect of IL-1αon IL-8and RANTES expression, sIL-1RⅡcan amend IL-1RⅠ/IL-1RⅡimbalance and lessen the immuno-inflammatory reaction of EMs.Maintaining the balance of IL-1RⅠ/IL-1RⅡmay be an original therapy method for endometriosis.