The Research Of The Value Of Biochemical Indicaters And Radionuclide Imaging In Early Diagnosis Of Rabbit Osteo-fascial Compartment Syndrome

Objectve To establish models of osoteo-fascial compartment syndrome(OFCS) in rabbits, and conduct dynamic study on the changes in blood biochemical indicators and radionuclide imaging, and analyze their effects in early diagnosis and evaluation of OFCS.Methods Fifteen Zelanian rabbits were established into OFCS models by strangulating one side lower leg of each rabbit with a tourniquet for eight hours and then loosed. The levels of serum phosphocreatine kinase (CPK), lactic dehydrogenase(LDH), aspartate aminotransferase(AST), malondialdehyde(MDA) and superoxidedismutase(SOD) were monitored dynamically before the experiment and 2, 12, 24, 48, 96, hours as well as 1 and 2 weeks after looseing the tournique. Meanwhile, intracompartment pressure(ICP) was monitored by using Whiteside method. 24 hours later, 85 MBq ⁹⁹T_c^m-PYP was injected through ear edge venous, and then quiet and delayed imaging were displayed using SPECT. Both sides of the lower leg were considered the region of interesting. The radioactive nuclide uptaking ratio of pressed leg/nomal leg was semiquantitative analyaed. The anterior tibial muscle and deep fibular nerve preparations were taken two weeks later for biopsy.Result A total of 15 rabbits were involved in the analysis of results. 1: Two hours later after loosing the tourniquet, the contents of serum phosphocreatine kinase, LDH, AST amd MDA increased dramatically, which were 20, 6, 6 and 2 times as those before the experiment, and they all peaked at the 24th hour, which were 37, 13, 12, and 3 times as those before as that before the experiment, One week after loosing the tourniquet, the concentrations of MDA and SOD recovered to the levels before the experiment, whereas other indicators were signficantly different from those before the experiment (P＜0.05). 2: After loosing the tourniquet, the injued legs began to swell quickly, and ICP increased to 4.0 kPa at 12 hours later, and peaked at the 24th hour[(7.3±0.4) kPa]. Meanwhile, T/NT in quiet and delayed imaging showed that the venous return was locked in pressed legs. 3: Pathological changes of muscle and nerve were irreversible.Conclusion Rabbit lower leg models of OFCS are successfully established, and the changes in phosphocreatine kinase, LDH, AST, SOD, and MDA objectively reflect the progression of disease. Dynamic and continuous monitoring of biochemical indicators can provide assistant for diagnosis of compartment syndrome and evaluating pathogenetic condition. Radionuclide imaging can also provide assistant for diagnosis of compartment syndrome.