Expressions Of COX-2,Ki-67 And Nm23 In Human Glioma And Their Clinical Significance

ObjectiveDetect expression of COX-2, Ki-67 in human gliomas and nm23. Research the relationship between the three and human glioma pathological classification and the correlation between the expressions. Explore the role of the three in the development of glioma.Methods(1) 10 cases of normal brain tissue. 56 cases of glioma specimens are classified on 2000 WHO nervous system tumor classification and grading standards for: Grade I , 12cases; Grade II ,16cases; Grade III ,16cases; Grade IV ,12cases. (2) S-P immunohistochemical staining was used to detect expression level of COX-2, Ki-67 and nm23 in glioma and normal brain tissue. (3) Statistical analysis: use rank sum test (Kraskal-wallis) and Spearman rank correlation analysis.α=0.05 is test criteria.ResultsThe positive expression of COX-2 wasn't observed in the normal brain tissue and the rates of positive expression of COX-2 in glioma Grade I , II group, Grade III group and Grade IV group were 25.00% , 75.00% and 100.00% respectively. The expression intensity in different glioma grade groups showed increasing trend by sequence, the difference was statistically significant (P<0.05). In further comparison of any two of them, it can be found that the difference between any two groups was statistically significant (P<0.05) only except the difference between Grade I group and Grade II group. (2) The rates of positive expression of Ki-67 in normal brain tissues, glioma Grade I , II group, Grade III group and Grade IV group were 0.00%, 42.86%, 56.25% and 83.33% respectively, the difference was statistically significant (P<0.05). In further comparison of any two of them, it can be found that the difference between any two groups was statistically significant (P<0.05) only except the difference between glioma Grade I , II group and Grade III group and the difference between Grade III group and Grade IV group. (3) The rates of positive expression of nm23 in normal brain tissues, glioma Grade I , II group, Grade III group and Grade IV group were 100.00%,78.57%, 37.50% and 16.67% respectively with the intensity of expression lowered by sequence, the difference was statistically significant (P<0.05). In further comparison of any two of them, it can be found that the difference between any two groups was statistically significant (P <0.05) only except the difference between Grade III group and Grade IV group. (4) Using Spearman rank correlation test, it can be confirmed that pathologic classification was positively correlated with COX-2 expression index (r_s=0.734, P <0.05) and Ki-67 expression index (r_s=0.483, P<0.05) and negatively correlated with nm23 expression index (r_s=-0.518, P<0.05), these were all highly significant (P <0.05); COX-2 expression was significantly positively correlated with Ki-67 expression in glioma (r_s=0.567. P<0.05). while nm23 and Ki-67 expressions were significantly negatively correlated (r_s=-0.388, P<0.05).ConclusionThe rates of positive expression of COX-2,Ki-67 and nm23 in normal brain tissues, glioma Grade I,II group, Grade III group and Grade IV group are different. Expression intensity of COX-2 and Ki-67 increases with the increase of degree of the malignant glioma; expression intensity of nm23 decreases with the increase of the degree of the malignant glioma. This indicates that these three may be involved in the development process of glioma. There is definite correlation between the positive expression of COX-2, Ki-67 and nm23 in glioma, which indicates that the development of glioma is a result of the interplay of many factors.