Effect Of Bone Marrow Mesenchymal Stem Cells On Acute Graft Versus Host Disease After Allogeneic Bone Marrow Transplantation

ObjectiveTo investigate the effect and the mechanism of bone marrow mesenchymal stem cells (BMMSC) on acute graft versus host disease(aGVHD) after allogeneic bone marrow transplantation (allo-BMT).MethodsIn the zero day,third day and seventh day after allo-BMT, BMMSC obtained after three to four weeks of culture from donor mice were transplanted into the TBI conditioned recipient mice, the control group was injected with bone marrow cells alone. The survial time after allo-BMT was recorded; the general manifestation and pathological changes of aGVHD and hematopoietic reconstitution in recipient mice were observed;the effects of BMMSC on the quantity of CD4~+ and CD8~+T cell in vivo after allo-BMT were evaluated by flow cytometry. The serum levels of both IFN-γand IL-4 were determined by ELISA.ResultsThe results showed BMMSC could obviously increase the survival time, and delay onset of aGVHD, BMMSC could increase the amount of CD4~+T cell and decrease CD8~+T cell in vivo. BMMSC could decrease the quantity of IL-4 and increase IFN-γin vivo, but this effect was decreased if BMMSC delay.ConclusionIt is concluded that cotransplantation of bone marrow cells with BMMSC from the same donor mice can alleviate aGVHD and the effect is best if both BM and BMMSC injected at same time, the mechanism is balancing the Thl/Th2-related cytokines after allo-BMT.